Compartir
Título
Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Research in Veterinary Science
Cita Bibliográfica
Lacalle, E., Martínez-Martínez, S., Fernández-Alegre, E., Soriano-Úbeda, C., Morrell, J. M., & Martínez-Pastor, F. (2023). Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species. Research in Veterinary Science, 158, 215–225. https://doi.org/10.1016/j.rvsc.2023.03.024
Editorial
Elsevier
Fecha
2023
ISSN
0034-5288
Resumen
[EN] Single-layer centrifugation (SLC) with a low-density colloid is an efficient method for removing contaminating microorganisms from boar semen while recovering most spermatozoa from the original sample. This study tested the performance of this technique, using 50-ml tubes, by spiking commercial semen doses prepared without antibiotics with selected bacterial species followed by storage at 17 °C. The doses were spiked up to 102/ml CFU (colony forming units) of the bacteria Burkholderia ambifaria, Pseudomonas aeruginosa, and Staphylococcus simulans. The semen was processed by SLC (15 ml of sample and 15 ml of colloid) with the colloid Porcicoll at 20% (P20) and 30% (P30), with a spiked control (CTL) and an unspiked control (CTL0), analyzing microbiology and sperm quality on days 0, 3 and 7. SLC completely removed B. ambifaria and S. simulans, considerably reducing P. aeruginosa and overall contamination (especially P30, ∼104 CFU/ml of total contamination on day 7, median). Sperm viability was lower in P20 and P30 samples at day 0, with higher cytoplasmic ROS. Still, results were similar in all groups on day 3 and reversed on day 7, indicating a protective effect of SLC (possibly directly by removal of damaged sperm and indirectly because of lower bacterial contamination). Sperm chromatin was affected by the treatment (lower DNA fragmentation and chromatin decondensation) and storage (higher overall condensation on day 7 as per chromomycin A3 and monobromobimane staining). In conclusion, SLC with low-density colloids can remove most bacteria in a controlled contamination design while potentially improving sperm quality and long-term storage at practical temperatures.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/AIE/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-095183-B-I00/ES/ INCREMENTO DE LA EFICIENCIA REPRODUCTIVA DE LA CRIA PORCINA MEDIANTE LA APLICACION DE TECNICAS AVANZADAS DE ANALISIS DE LA CROMATINA ESPERMATICA Y SELECCION POR COLOIDE
- Junta de Castilla y León/LE023P20
URI
DOI
Aparece en las colecciones
- Artículos [4950]
Ficheros en el ítem
Tamaño:
4.403
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF