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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Ibáñez Sánchez, Ana María | |
dc.contributor.author | Barreiro Méndez, Carlos | |
dc.contributor.author | Diez Galán, Alba | |
dc.contributor.author | Cobos Román, Rebeca | |
dc.contributor.author | Calvo Peña, Carla | |
dc.contributor.author | Rubio Coque, Juan José | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2023-08-29 | |
dc.date.accessioned | 2024-02-02T07:36:15Z | |
dc.date.available | 2024-02-02T07:36:15Z | |
dc.identifier.citation | Ibáñez, A., Barreiro, C., Diez-Galán, A., Cobos, R., Calvo-Peña, C., & Coque, J. J. R. (2023). Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain). International Journal of Molecular Sciences, 24(17). https://doi.org/10.3390/IJMS241713391 | es_ES |
dc.identifier.other | https://www.mdpi.com/1422-0067/24/17/13391 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18007 | |
dc.description.abstract | [EN] Acidithiobacillus thiooxidans is of paramount importance in the development of biomining technologies. Being widely recognized as an extreme acidophile, extensive research has been dedicated to understanding its significant role in the extraction of several ores in recent years. However, there still exist significant molecular uncertainties surrounding this species. This study focuses on developing a taxonomic assignment method based on the sequencing of the 16S-5S rRNA cluster, along with a qPCR-based technology enabling precise growth determination. Additionally, an approach to understanding its response to acid stress is explored through RT-PCR and MALDITOF analysis. Our findings indicate that when subjected to pH levels below 1, the cell inhibits central (carbon fixation and metabolism) and energy (sulfur metabolism) metabolism, as well as chaperone synthesis, suggesting a potential cellular collapse. Nevertheless, the secretion of ammonia is enhanced to raise the environmental pH, while fatty acid synthesis is upregulated to reinforce the cell membrane. | es_ES |
dc.language | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Biología | es_ES |
dc.subject.other | Acidithiobacillus thiooxidans | es_ES |
dc.subject.other | 2D-DIGE | es_ES |
dc.subject.other | RT-PCR | es_ES |
dc.subject.other | pH stress | es_ES |
dc.subject.other | Omics | es_ES |
dc.subject.other | Mass spectrometry | es_ES |
dc.title | Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain) | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3390/ijms241713391 | |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1422-0067 | |
dc.journal.title | International Journal of Molecular Sciences | es_ES |
dc.volume.number | 24 | es_ES |
dc.issue.number | 17 | es_ES |
dc.page.initial | 13391 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2415 Biología Molecular | es_ES |
dc.subject.unesco | 2511.10 Mineralogía de suelos | es_ES |
dc.subject.unesco | 2403 Bioquímica | es_ES |
dc.description.project | We thank Instituto de Biotecnología de León (INBIOTEC) for their technical support in performing the A. thiooxidans proteome analysis. Special thanks to (i) the ESTELLA project (“DESign of bio-based Thermoset polymer with rEcycLing capabiLity by dynAmic bonds for bio-composite manufacturing”) (Project no.: 101058371) funded by the European Union through the Horizon Europe Framework Programme (call: HORIZON-CL4-2021-RESILIENCE-01-11) and (ii) the BioPAC project (Development of bioactive and lifespan-controlled bioplastics) (Ref. TED2021-131864B-C21) funded by the MCIN (Ministerio de Ciencia e Innovación)/AEI (Agencia Estatal de Investigación)/10.13039/501100011033 (DOI) and the European Union “NextGenerationEU”/PRTR (Plan de Recuperación, Transformación y Resiliencia). | es_ES |
dc.description.project | Ana Ibañez was supported by a “Margarita Salas” modality postdoctoral grant (Reference no.: UP2021-025) through the University of León awarded by the Spanish Ministry of Universities within the Recovery, Transformation and Resilience Plan (Modernization and digitalization of the Educational System), which funding comes from the European Recovery Instrument European Union-NextGeneration EU. Alba Diez-Galán (EDU/556/2019), and Carla Calvo-Peña (EDU/601/2020) were supported by a predoctoral contract from the Junta de Castilla y León and the European Social Fund. | es_ES |
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