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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Martínez Burgo, Yolanda | |
dc.contributor.author | Santos Aberturas, Javier | |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | García Barreales, Eva | |
dc.contributor.author | Tormo, José Rubén | |
dc.contributor.author | Truman, Andrew W. | |
dc.contributor.author | Reyes, Fernando | |
dc.contributor.author | Aparicio Fernández, Jesús Manuel | |
dc.contributor.author | Liras Padín, Paloma | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2019-03-26 | |
dc.date.accessioned | 2024-02-06T07:21:55Z | |
dc.date.available | 2024-02-06T07:21:55Z | |
dc.identifier.citation | Martínez-Burgo, Y., Santos-Aberturas, J., Rodríguez-García, A., Barreales, E. G., Tormo, J. R., Truman, A. W., Reyes, F., Aparicio, J. F., & Liras, P. (2019). Activation of secondary metabolite gene clusters in Streptomyces clavuligerus by the PimM regulator of Streptomyces natalensis. Frontiers in microbiology, 10, Article e580. https://doi.org/10.3389/fmicb.2019.00580 | es_ES |
dc.identifier.other | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00580/full | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18075 | |
dc.description.abstract | [EN] Expression of non-native transcriptional activators may be a powerful general method to activate secondary metabolites biosynthetic pathways. PAS-LuxR regulators, whose archetype is PimM, activate the biosynthesis of polyene macrolide antifungals and other antibiotics, and have been shown to be functionally preserved across multiple Streptomyces strains. In this work we show that constitutive expression of pimM in Streptomyces clavuligerus ATCC 27064 significantly affected its transcriptome and modifies secondary metabolism. Almost all genes in three secondary metabolite clusters were overexpressed, including the clusters responsible for the biosynthesis of the clinically important clavulanic acid and cephamycin C. In comparison to a control strain, this resulted in 10- and 7-fold higher production levels of these metabolites, respectively. Metabolomic and bioactivity studies of S. clavuligerus::pimM also revealed deep metabolic changes. Antifungal activity absent in the control strain was detected in S. clavuligerus::pimM, and determined to be the result of a fivefold increase in the production of the tunicamycin complex | es_ES |
dc.language | eng | es_ES |
dc.publisher | Frontiers Media SA | es_ES |
dc.subject | Biotecnología | es_ES |
dc.subject.other | Streptomyces clavuligerus | es_ES |
dc.subject.other | Antibotics biosynthesis regulation | es_ES |
dc.subject.other | PimM | es_ES |
dc.subject.other | Clavulanic acid | es_ES |
dc.subject.other | Cephamycin C | es_ES |
dc.subject.other | Transcriptomics | es_ES |
dc.title | Activation of Secondary Metabolite Gene Clusters in Streptomyces clavuligerus by the PimM Regulator of Streptomyces natalensis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3389/fmicb.2019.00580 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2013-34723 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1664-302X | |
dc.journal.title | Frontiers in Microbiology | es_ES |
dc.volume.number | 10 | es_ES |
dc.page.initial | 580 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414.01 Antibióticos | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.description.project | This work was supported by grant BIO2013-34723 from the Spanish Ministry of Economy and Competitiveness (to PL); by BIO2013-42983-P and PCIN-2016-190 from MINEICO (to JA); and the John Innes Centre Molecules from Nature Institute Strategic Programme (to AT). YM-B received a FPU fellowship from the Spanish Ministry of Science and Innovation | es_ES |
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