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Título
Identification of molecular biomarkers associated with disease progression in the testis of bulls infected with Besnoitia besnoiti
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Veterinary Research
Número de la revista
1
Cita Bibliográfica
González-Barrio, D., Diezma-Díaz, C., Gutiérrez-Expósito, D., Tabanera, E., Jiménez-Meléndez, A., Pizarro, M., González-Huecas, M., Ferre, I., Ortega-Mora, L. M., & Álvarez-García, G. (2021). Identification of molecular biomarkers associated with disease progression in the testis of bulls infected with Besnoitia besnoiti. Veterinary research, 52(1), 106. https://doi.org/10.1186/S13567-021-00974-2
Editorial
BMC
Fecha
2021
Resumen
[EN]Breeding bulls infected with Besnoitia besnoiti may develop sterility during either acute or chronic infection. The aim of this study was to investigate the molecular pathogenesis of B. besnoiti infection with prognosis value in bull sterility. Accordingly, five well-characterized groups of naturally and experimentally infected males were selected for the study based on clinical signs and lesions compatible with B. besnoiti infection, serological results and parasite detection. A broad panel of molecular markers representative of endothelial activation and fibrosis was investigated and complemented with a histopathological approach that included conventional histology and immunohistochemistry. The results indicated the predominance of an intense inflammatory infiltrate composed mainly of resident and recruited circulating macrophages and to a lesser extent of CD3+ cells in infected bulls. In addition, a few biomarkers were associated with acute, chronic or subclinical bovine besnoitiosis. The testicular parenchyma showed a higher number of differentially expressed genes in natural infections (acute and chronic infections) versus scrotal skin in experimental infections (subclinical infection). In subclinical infections, most genes were downregulated except for the CCL24 and CXCL2 genes, which were upregulated. In contrast, the acute phase was mainly characterized by the upregulation of IL-1α, IL-6 and TIMP1, whereas in the chronic phase, the upregulation of ICAM and the downregulation of MMP13, PLAT and IL-1α were the most relevant findings. Macrophages could be responsible for the highest level of gene regulation in the testicular parenchyma of severely affected and sterile bulls, and all these genes could be prognostic markers of sterility.
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- info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-103960RB-I00/ES/CONTROL QUIMIOTERAPEUTICO DE LA BESNOITIOSIS BOVINA
- info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES-2014-069839
- info:eu-repo/grantAgreement/MECD/Programa Estatal de Promoción del Talento y su Empleabilidad/FPU13/05481
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