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Título
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Veterinary Medicine and Science
Número de la revista
1
Cita Bibliográfica
Infantes-Lorenzo, J. A., Dave, D., Moreno, I., Anderson, P., Lesellier, S., Gormley, E., Dominguez, L., Balseiro, A., Gortázar, C., Dominguez, M., & Salguero, F. J. (2019). New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers. Veterinary Medicine and Science, 5(1), 61-69. https://doi.org/10.1002/VMS3.134
Editorial
Wiley Open Access
Fecha
2019
ISSN
2053-1095
Resumen
[EN]European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis-infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (PPD) of M. bovis. The recognition of IgG against P22 multiprotein complex derived from PPD-B was tested by ELISA in the serum of badgers from the UK, Ireland and Spain. TB infection in the badgers was indicated by the presence of M. bovis in tissues by culture and histology at post-mortem examination and TB-free status was established by repeated negativity in the interferon c release assay (IGRA). In experimentally infected badgers, humoral antibody responses against P22 developed within 45 days post-infection. The ELISA tests showed estimated sensitivity levels of 74–82% in experimentally and naturally infected badgers with specifici-ties ranging from 75% to 100% depending on the badger population tested. The P22 multi-antigen based ELI-SAs provide a sensitive and specific test platform for improved tuberculosis surveillance in badgers.
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Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00002-C02-01/ES/Estrategias de control de la tuberculosis bovina en reservorios silvestres (tejón y jabalí) y domésticos (ovino)
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