Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma

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Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma

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Title: Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma
Author: Fernández, Nélida;García, Juan J.;Diez, M. José;Terán, M. Teresa;Sierra, Matilde
xmlui.dri2xhtml.METS-1.0.item-contributor: Facultad de Veterinaria
xmlui.dri2xhtml.METS-1.0.item-area: Farmacologia
Abstract: A method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phase high-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of + 1 V vs. Ag/AgCI. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min , the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 ml of plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.
xmlui.dri2xhtml.METS-1.0.item-desfisica: P. 143-147
xmlui.dri2xhtml.METS-1.0.item-peerreviewed: SI
Publisher: Elsevier
xmlui.dri2xhtml.METS-1.0.item-citation: Journal of Chromatography, 1993, n. 619
URI: http://hdl.handle.net/10612/4574
Date: 1993-05-28
xmlui.dri2xhtml.METS-1.0.item-tipo: info:eu-repo/semantics/article
Subject: Ecología. Medio ambiente
Farmacología
Veterinaria
xmlui.dri2xhtml.METS-1.0.item-palclave: Chromatographic
Ethinylestradiol
Rabbit plasma
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