Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma

Repositorio Dspace/Manakin

Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma

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Título: Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma
Autor: Fernández, Nélida;García, Juan J.;Diez, M. José;Terán, M. Teresa;Sierra, Matilde
Facultad/Centro: Facultad de Veterinaria
Area de conocimiento: Farmacologia
Resumen: A method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phase high-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of + 1 V vs. Ag/AgCI. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min , the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 ml of plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.
Descripción física: P. 143-147
Revisión por pares: SI
Editor: Elsevier
Datos: Journal of Chromatography, 1993, n. 619
URI: http://hdl.handle.net/10612/4574
Fecha: 1993-05-28
Tipo: info:eu-repo/semantics/article
Materia: Ecología. Medio ambiente
Farmacología
Veterinaria
Palabras clave: Chromatographic
Ethinylestradiol
Rabbit plasma
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