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Título
Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma
Autor
Facultad/Centro
Área de conocimiento
Cita Bibliográfica
Journal of Chromatography, 1993, n. 619
Editorial
Elsevier
Fecha
1993-05-28
Resumen
A method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phase
high-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of + 1 V vs. Ag/AgCI. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min , the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 ml
of plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.
Materia
Palabras clave
Peer review
SI
URI
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