Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces

Repositorio Dspace/Manakin

Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces

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Título: Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces
Autor: Villar-Suárez, V.;Calles-Venal, I.;Bravo, G.;Fernández Álvarez, José Gabriel;Fernández-Caso, M.;Villar-Lacilla, J. M.
Facultad/Centro: Facultad de Ciencias Biologicas y Ambientales
Area de conocimiento: Bioquimica y Biologia Molecular
Resumen: A knowledge of the behavior of chondrocytes in culture is relevant for tissue engineering. Chondrocytes dedifferentiate 10 a fibroblast· like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured in suspension. In this report a descrip· tion is given of how when chondrocyte aggregates formed in suspension are next seeded on plastic, most of them attach as round or polygonal cells. This morphological differentiation, with synthesis of type II collagen, is stable for long culture periods. This sim· pie method can be of use as a model for studies of chondrocyte behavior on plastic. The results indicate that in addition to culture conditions, such as cell isolation method or cell density, chondrocyte behavior on plastic depends on the presence of aggregates
Descripción física: P. 86-92
Revisión por pares: SI
Editor: Hindawi Publishing Corporation
Datos: Journal of Biomedicine and Biotechnology, 2004, n. 2
URI: http://hdl.handle.net/10612/6447
Fecha: 2004-01-23
Tipo: info:eu-repo/semantics/article
Materia: Bioquímica
Biotecnología
Palabras clave: Auricular Chondrocytes
Plastic Surfaces
Behavior
Rabbits
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