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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Fregeneda Grandes, Juan Miguel | |
dc.contributor.author | Rodríguez Cadenas, Fausto | |
dc.contributor.author | Carbajal González, María Teresa | |
dc.contributor.author | Aller Gancedo, José Miguel | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2007-02-28 | |
dc.date.accessioned | 2018-05-27T20:54:24Z | |
dc.date.available | 2018-05-27T20:54:24Z | |
dc.date.issued | 2018-05-27 | |
dc.identifier.citation | Diseases of aquatic organisms, 2007, vol. 74, n. 2 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/8019 | |
dc.description | P. 107-111 | es_ES |
dc.description.abstract | Brown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion (ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia grown in medium with or without β-sytosterol (SEB and SE, respectively). In the 2 groups injected with SE and SEB, antibodies were found in 66.7% of the serum samples by ELISA, 54.5% by IF and 48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected with SE or SEB. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Inter-Research | es_ES |
dc.subject | Inmunología | es_ES |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject | Zoología | es_ES |
dc.subject.other | Truchas | es_ES |
dc.subject.other | Saprolegnia parasítica | es_ES |
dc.subject.other | Inmunoglobulinas | es_ES |
dc.title | Antibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extracts | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
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