RT info:eu-repo/semantics/article
T1 Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa
A1 Nicolás, M.
A1 Álvarez García, Mercedes
A1 Barragán Santos, Santiago
A1 Martínez Pastor, Felipe
A1 Chamorro Álvarez, César Ángel
A1 Álvarez Rodríguez, Manuel
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Centrifugation
K1 Density gradient
K1 Cryopreservation
K1 Brown bear
K1 Spermatozoa
AB Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample.
PB Elsevier
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10612/10673
UL http://hdl.handle.net/10612/10673
NO Theriogenology, 2012,  vol. 77, n. 6
NO P. 1119-1128
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 03-jun-2024