RT info:eu-repo/semantics/article
T1 Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil
A1 Polanco de la Puente, Carlos Gaspar
A1 Sáenz de Miera Carnicer, Luis Enrique
A1 González Cordero, Ana Isabel
A1 García García, Pedro
AD  1957-
A1 Fratini, Richard
A1 Vaquero Rodrigo, Francisca
A1 Vences Benito, Francisco Javier
A1 Pérez de la Vega, Marcelino
A2 Genetica
K1 Genética
K1 Mapas genéticos
K1 2409 Genética
AB Usage of high-throughput sequencing approaches allow for the generation and characterizationof reference transcriptome datasets that support gene-based marker discovery,which in turn can be used to build genetic maps among other purposes. We have obtained atranscriptome assembly including 49,453 genes for the lentil (Lens culinaris Medik.) cultivarAlpo using RNAseq methodology. This transcriptome was used as reference to obtain6,306 quality polymorphic markers (SNPs and short indels) analyzing genotype data from aRIL population at F7 generation derived from the interspecific cross between L. culinaris cv.Alpo and L. odemensis accession ILWL235. L. odemensis is a wild species included in thesecondary gene pool and can be used as a source for gene introgression in lentil breedingprograms. Marker data were used to construct the first genetic interspecific map betweenthese two species. This linkage map has been used to precisely identify regions of the CDCRedberrylentil draft genome in which the candidate genes for some qualitative traits (seedcoat spotting pattern, flower color, and stem pigmentation) could be located. The genomeregions corresponding to a significant single quantitative trait locus (QTL) controlling “timeto flowering” located in chromosome 6 and three QTLs regulating seed size and positionedin chromosomes 1 and 5 (two QTLs) were also identified. Significant QTLs for Ascochytablight resistance in lentil were mapped to chromosome 6 in the genome region or close to itwhere QTLs for Ascochyta blight resistance have previously been reported.
PB PLoS
LK http://hdl.handle.net/10612/11115
UL http://hdl.handle.net/10612/11115
NO PLoS ONE vol. 14, n. 3
NO Revista electrónica on line
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024