RT info:eu-repo/semantics/article
T1 Stress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny
A1 García Valcarce, David
A1 Fernández Riesco, Marta
A1 Cuesta Martín, Leyre
A1 Esteve Codina, Anna
A1 Martínez-Vázquez, Juan Manuel
A1 Robles Rodríguez, Vanesa
A2 Bioquimica y Biologia Molecular
K1 Biología
K1 Zebrafish
K1 Chronic stress
K1 Testis
K1 NMD
K1 Sperm
K1 Progeny
K1 2415 Biología Molecular
AB [EN] Background Chronic stress can produce a severe negative impact on health not only in the exposed individualsbut also in their offspring. Indeed, chronic stress may be contributing to the current worldwide scenario of increasinginfertility and decreasing gamete quality in human populations. Here, we evaluate the effect of chronic stresson behavior and male reproductive parameters in zebrafish. Our goal is to provide information on the impact thatchronic stress has at molecular, histological, and physiological level in a vertebrate model species.Results We evaluated the effects of a 21-day chronic stress protocol covering around three full waves of spermatogenesisin Danio rerio adult males. The induction of chronic stress produced anxiety-like behavior in stressed males asassessed by a novel tank test. At a molecular level, the induction of chronic stress consistently resulted in the overexpressionof two genes related to endoplasmic reticulum (ER) stress in the brain. Gene set enrichment analysis (GSEA)of testes suggested a dysregulation of the nonsense-mediated decay (NMD) pathway, which was also confirmed onqPCR analysis. Histological analysis of the testicle did not show significant differences in terms of the relative proportionsof each germ-cell type; however, the quality of sperm from stressed males was compromised in terms of motility.RNA-seq analysis in stress-derived larval progenies revealed molecular alterations, including those predicted toaffect translation initiation, DNA repair, cell cycle control, and response to stress.Conclusions Induction of chronic stress during a few cycles of spermatogenesis in the vertebrate zebrafish modelaffects behavior, gonadal gene expression, final gamete quality, and progeny. The NMD surveillance pathway (a keycellular mechanism that regulates the stability of both normal and mutant transcripts) is severely affected in the testesby chronic stress and therefore the control and regulation of RNAs during spermatogenesis may be affected alteringthe molecular status in the progeny.
PB Springer Nature
LK https://hdl.handle.net/10612/17463
UL https://hdl.handle.net/10612/17463
NO Valcarce, D.G., Riesco, M.F., Cuesta-Martín, L., Esteve-Codina, A., Martínez-Vájuez, J.M., Robles, V. (2023). Stress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny. 21, 70 (2023). https://doi.org/10.1186/s12915-023-01570-w
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024