RT info:eu-repo/semantics/article
T1 Characterization and nitrogen-source regulation at the transcriptional level of the gdhA gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase
A1 Cardoza, Rosa E.
A1 Moralejo, Francisco J.
A1 Gutiérrez Martín, Santiago
A1 Casqueiro Blanco, Francisco Javier
A1 Fierro, Francisco
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Glutamate dehydrogenase
K1 Nitrogen regulation
K1 Asperillus awamori
K1 Chromosome resolution
K1 Promoter
K1 2414 Microbiología
K1 2414.06 Hongos
K1 2414.07 Metabolismo Microbiano
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] A 28.7-kb DNA region containing the gdhA gene of Aspergillus awamori was cloned from a genomic DNA library. A fragment of 2570 nucleotides was sequenced that contained ORF1, of 1380 bp, encoding a protein of 460 amino acids (Mr 49.4 kDa). The encoded protein showed high similarity to the NADP-dependent glutamate dehydrogenases of different organisms. The cloned gene was functional since it complemented two different Aspergillus nidulans gdhA mutants, restoring high levels of NADP-dependent glutamate dehydrogenase to the transformants. The A. awamori gdhA gene was located by pulsed-field gel electrophoresis in a 5.5-Mb band (corresponding to a doublet of chromosomes II and III), and was transcribed as a monocistronic transcript of 1.7 kb. Transcript levels of the gdhA gene were very high during the rapid growth phase and decreased drastically after 48 h of cultivation. Very high expression levels of the gdhA gene were observed in media with ammonium or asparagine as the nitrogen source, whereas glutamic acid repressed transcription of the gdhA gene. These results indicate that expression of the gdhA gene is subject to a strong nitrogen regulation at the transcriptional level.
PB Springer Link
SN 0172-8083
LK https://hdl.handle.net/10612/17816
UL https://hdl.handle.net/10612/17816
NO Cardoza, R. E., Moralejo, F., Gutiérrez, S., Casqueiro, J., Fierro, F., y Martı́n, J. F. (1998b). Characterization and nitrogen-source regulation at the transcriptional level of the GDH A gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase. Current Genetics, 34(1), 50-59. https://doi.org/10.1007/s002940050365
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024