RT info:eu-repo/semantics/article
T1 Intralaboratory Validation of a Kinetic Turbidimetric Assay Based on Limulus Amebocyte Lysate (LAL) for Assessing Endotoxin Activity in Cow Milk
A1 Flórez, Pablo
A1 Castro, María de
A1 Rodríguez, David
A1 Gonzalo Orden, José Manuel
A1 Carvajal Urueña, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Cattle
K1 Endotoxin
K1 limulus amebocyte lysate
K1 Milk
K1 Mastitis
K1 Lipopolysaccharide
K1 3109 Ciencias Veterinarias
AB [EN] Mastitis, one of the most common diseases in dairy cattle, causes severe losses in the dairy sector worldwide and affects animal welfare. The disease is characterized by an inflammatory reaction of the mammary gland and is mainly caused by bacterial infections, including both Gram-negative and Gram-positive bacteria. The release of endotoxins associated to bacterial lysis is a weighty factor in the clinical course of Gram-negative associated mastitis and should be taken into consideration when using antibiotics in the treatment of these infections. Therefore, endotoxin detection in milk samples would be of help in the management of bovine mastitis. With this aim, we have validated a kinetic turbidimetric assay based on Limulus amebocyte lysate (LAL) for the quantification of endotoxins in milk samples. The assay was adapted to this particular matrix by incorporating filtration and dilution of the milk samples in the procedure. Our results demonstrate the robustness and usefulness of the assay, which allows the identification of coliform mastitis in milk samples from affected cows and the quantification of endotoxin activity in bulk and commercial milk samples. Further studies are required to evaluate the performance of the assay in mastitis milk samples associated to Gram-negative bacteria other than Escherichia coli as well as during the clinical course of these Gram-negative mastitis or after their treatment with antibiotics.
PB MDPI
LK https://hdl.handle.net/10612/17883
UL https://hdl.handle.net/10612/17883
NO Flórez, P., de Castro, M., Rodríguez, D., Gonzalo-Orden, J. M., & Carvajal, A. (2023). Intralaboratory Validation of a Kinetic Turbidimetric Assay Based on Limulus Amebocyte Lysate (LAL) for Assessing Endotoxin Activity in Cow Milk. Animals, 13(3). https://doi.org/10.3390/ANI13030427
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024