RT info:eu-repo/semantics/article
T1 Characterization and cross‐protection of experimental infections with SeCoV and two PEDV variants
A1 Puente Fernández, Héctor
A1 Díaz, Iván
A1 Argüello Rodríguez, Héctor
A1 Mencía Ares, Óscar
A1 Gómez García, Manuel
A1 Pérez Pérez, Lucía
A1 Vega, Clara
A1 Cortey, Martí
A1 Martín, Margarita
A1 Rubio Nistal, Pedro Miguel
A1 Carvajal Urueña, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Immunity
K1 Porcine epidemic diarrhoea virus
K1 Recombinant
K1 Swine coronavirus
K1 Swine enteric coronavirus
K1 3109 Ciencias Veterinarias
AB [EN] The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) – a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhoea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus – and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV. For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals. Piglets within each group were primary inoculated with one of the investigated viral strains (B: PEDV; C: SeCoV and D: rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored. After primo-infection, all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhoea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in faeces
PB Wiley
SN 1865-1674
LK https://hdl.handle.net/10612/18006
UL https://hdl.handle.net/10612/18006
NO Puente, H., Díaz, I., Arguello, H., Mencía-Ares, Ó., Gómez-García, M., Pérez-Pérez, L., Vega, C., Cortey, M., Martín, M., Rubio, P., & Carvajal, A. (2022). Characterization and cross-protection of experimental infections with SeCoV and two PEDV variants. Transboundary and Emerging Diseases, 69(6), 3225-3237. https://doi.org/10.1111/TBED.14674
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024