RT info:eu-repo/semantics/article
T1 Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain)
A1 Ibáñez Sánchez, Ana María
A1 Barreiro Méndez, Carlos
A1 Diez Galán, Alba
A1 Cobos Román, Rebeca
A1 Calvo Peña, Carla
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Acidithiobacillus thiooxidans
K1 2D-DIGE
K1 RT-PCR
K1 pH stress
K1 Omics
K1 Mass spectrometry
K1 2415 Biología Molecular
K1 2511.10 Mineralogía de suelos
K1 2403 Bioquímica
AB [EN] Acidithiobacillus thiooxidans is of paramount importance in the development of biomining technologies. Being widely recognized as an extreme acidophile, extensive research has been dedicated to understanding its significant role in the extraction of several ores in recent years. However, there still exist significant molecular uncertainties surrounding this species. This study focuses on developing a taxonomic assignment method based on the sequencing of the 16S-5S rRNA cluster, along with a qPCR-based technology enabling precise growth determination. Additionally, an approach to understanding its response to acid stress is explored through RT-PCR and MALDITOF analysis. Our findings indicate that when subjected to pH levels below 1, the cell inhibits central (carbon fixation and metabolism) and energy (sulfur metabolism) metabolism, as well as chaperone synthesis, suggesting a potential cellular collapse. Nevertheless, the secretion of ammonia is enhanced to raise the environmental pH, while fatty acid synthesis is upregulated to reinforce the cell membrane.
PB MDPI
LK https://hdl.handle.net/10612/18007
UL https://hdl.handle.net/10612/18007
NO Ibáñez, A., Barreiro, C., Diez-Galán, A., Cobos, R., Calvo-Peña, C., & Coque, J. J. R. (2023). Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain). International Journal of Molecular Sciences, 24(17). https://doi.org/10.3390/IJMS241713391
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024