RT info:eu-repo/semantics/article
T1 Activation of Secondary Metabolite Gene Clusters in Streptomyces clavuligerus by the PimM Regulator of Streptomyces natalensis
A1 Martínez Burgo, Yolanda
A1 Santos Aberturas, Javier
A1 Rodríguez García, Antonio
A1 García Barreales, Eva
A1 Tormo, José Rubén
A1 Truman, Andrew W.
A1 Reyes, Fernando
A1 Aparicio Fernández, Jesús Manuel
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biotecnología
K1 Streptomyces clavuligerus
K1 Antibotics biosynthesis regulation
K1 PimM
K1 Clavulanic acid
K1 Cephamycin C
K1 Transcriptomics
K1 2414.01 Antibióticos
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Expression of non-native transcriptional activators may be a powerful general method to activate secondary metabolites biosynthetic pathways. PAS-LuxR regulators, whose archetype is PimM, activate the biosynthesis of polyene macrolide antifungals and other antibiotics, and have been shown to be functionally preserved across multiple Streptomyces strains. In this work we show that constitutive expression of pimM in Streptomyces clavuligerus ATCC 27064 significantly affected its transcriptome and modifies secondary metabolism. Almost all genes in three secondary metabolite clusters were overexpressed, including the clusters responsible for the biosynthesis of the clinically important clavulanic acid and cephamycin C. In comparison to a control strain, this resulted in 10- and 7-fold higher production levels of these metabolites, respectively. Metabolomic and bioactivity studies of S. clavuligerus::pimM also revealed deep metabolic changes. Antifungal activity absent in the control strain was detected in S. clavuligerus::pimM, and determined to be the result of a fivefold increase in the production of the tunicamycin complex
PB Frontiers Media SA
LK https://hdl.handle.net/10612/18075
UL https://hdl.handle.net/10612/18075
NO Martínez-Burgo, Y., Santos-Aberturas, J., Rodríguez-García, A., Barreales, E. G., Tormo, J. R., Truman, A. W., Reyes, F., Aparicio, J. F., & Liras, P. (2019). Activation of secondary metabolite gene clusters in Streptomyces clavuligerus by the PimM regulator of Streptomyces natalensis. Frontiers in microbiology, 10, Article e580. https://doi.org/10.3389/fmicb.2019.00580
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024