RT info:eu-repo/semantics/article T1 Morphological and ultrasonographic characterization of the three zones of supratesticular region of testicular artery in Assaf rams A1 Hassan, Mohamed A. A. A1 Sayed, Ramy K.A. A1 Abdelsabour-Khalaf, Mohammed A1 Abd-Elhafez, Enas A. A1 Anel López, Luis A1 Fernández Riesco, Marta A1 Ortega Ferrusola, Cristina A1 Montes Garrido, Rafael A1 Neila Montero, Marta A1 Anel Rodríguez, Luis A1 Álvarez García, Mercedes A2 Medicina y Cirugia Animal K1 Veterinaria K1 ovine K1 ultrasound K1 testicular artery K1 Doppler K1 3109 Ciencias Veterinarias AB [EN] To fully understand the histological, morphometrical and heamodynamic variations of different supratesticular artery regions, 20 mature and healthy Assaf rams were examined through ultrasound and morphological studies. The testicular artery images of the spermatic cord as shown by B-mode analysis indicated a tortuous pattern along its course toward the testis, although it tends to be less tortuous close to the inguinal ring. Doppler velocimetric values showed a progressive decline in flow velocity, in addition to pulsatility and vessel resistivity when entering the testis, where there were significant differences in the Doppler indices and velocities among the different regions. The peak systolic velocity, pulsatility index and resistive index were higher in the proximal supratesticular artery region, followed by middle and distal ones, while the end diastolic velocity was higher in the distal supratesticular region. The total arterial blood flow and total arterial blood flow rate reported a progressive and significant increase along the testicular cord until entering the testis. Histological examination revealed presence of vasa vasorum in the tunica adventitia, with their diameter is higher in the proximal supratesticular zone than middle and distal ones. Morphometrically, the thickness of the supratesticular artery wall showed a significant decline downward toward the testis; meanwhile, the outer arterial diameter and inner luminal diameter displayed a significant increase distally. The expression of alpha smooth muscle actin and vimentin was higher in the tunica media of the proximal supratesticular artery zone than in middle and distal ones. PB Nature Portfolio LK https://hdl.handle.net/10612/18283 UL https://hdl.handle.net/10612/18283 NO Hassan, M. A., Sayed, R. K., Abdelsabour-Khalaf, M., Abd-Elhafez, E. A., Anel-Lopez, L., Riesco, M. F., ... & Alvarez, M. (2022). Morphological and ultrasonographic characterization of the three zones of supratesticular region of testicular artery in Assaf rams. Scientific Reports, 12(1), 8334. DS BULERIA. Repositorio Institucional de la Universidad de León RD 20-may-2024