RT info:eu-repo/semantics/article
T1 Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
A1 Anel López, Luis
A1 Ortega Ferrusola, Cristina
A1 Martínez Rodríguez, C.
A1 Álvarez García, Mercedes
A1 Borragán Santos, Santiago
A1 Chamorro Álvarez, César Ángel
A1 Peña Vega, Fernando Juan
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Sanidad Animal
K1 Veterinaria
K1 Seminal plasma
K1 Brown bear
K1 Ursus arctos
K1 Testosterone
AB [EN] Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum during the breeding season. Forty-eight sperm samples from 30 sexually mature male brown bears (Ursus arctos) were obtained by electroejaculation, and their serum testosterone levels were assessed to sort the animals into 2 groups (high and low testosterone levels, threshold 5 ng/dl). The biochemical and protein compositions of the SP samples were assessed, and sperm motility was analyzed. We found that lactate dehydrogenase was significantly higher in the low-serum-testosterone samples, while concentrations of lipase and Mg+ values were significantly higher in the high-serum-testosterone samples. In contrast, sperm motility did not significantly differ (P>0.05) between the testosterone level groups (total motility: 74.42.8% in the high-level group vs. 77.1±4.7% in the low-level group). A reference digital model was constructed since there is no information for this wild species. To do this, all gel images were added in a binary multidimensional image and thirty-three spots were identified as the most-repeated spots. An analysis of these proteins was done by qualitative equivalency (isoelectric point and molecular weight) with published data for a bull. SP protein composition was compared between bears with high and low serum testosterone, and three proteins (binder of sperm and two enzymes not identified in the reference bull) showed significant (P<0.05) quantitative differences. We conclude that male bears with high or low serum testosterone levels differs only in some properties of their SP, differences in enzyme LDIP2, energy source LACT2, one protein (similar to BSP1) and Mg ion were identified between these two groups. These data may inform the application of SP to improve bear semen extenders.
PB Public Library of Sciene
LK https://hdl.handle.net/10612/18476
UL https://hdl.handle.net/10612/18476
NO Anel-López, Ortega-Ferrusola, Martínez-Rodríguez, Álvarez, Borragán, Chamorro, Peña, Anel, & De Paz. (2017). Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. PLoS ONE, 12(8). https://doi.org/10.1371/JOURNAL.PONE.0181776
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024