RT info:eu-repo/semantics/article
T1 Development and Evaluation of a Disease Large Animal Model for Preclinical Assessment of Renal Denervation Therapies
A1 Pérez de Prado, Armando
A1 Pérez Martínez, Claudia
A1 Regueiro Purriños, Marta María
A1 Cuellas Ramón, Carlos
A1 López Benito, María
A1 Gonzalo Orden, José Manuel
A1 Estévez Loureiro, Rodrigo
A1 Cortina Rivero, Ana Isabel
A1 Viñuela Baragaño, David
A1 Rodríguez-Áltonaga Martínez, José Antonio
A1 Tellez, Armando
A1 Fernández Vázquez, Felipe
A2 Medicina y Cirugia Animal
K1 Veterinaria
K1 Hypertension
K1 DOCA
K1 Renal denervation
K1 Radiofrequency
K1 Swine
K1 Histology
K1 Immunohistochemistry
K1 3109 Ciencias Veterinarias
AB [EN] New-generation catheters-based renal denervation (RDN) is under investigation for the treatment of uncontrolled hypertension (HTN). We assessed the feasibility of a large animal model of HTN to accommodate the human RDN devices. Ten minipigs were instrumented to measure blood pressure (BP) in an awake-state. HTN was induced with subcutaneous 11-deoxycorticosterone (DOCA, 100 mg/kg) implants. Five months after, the surviving animals underwent RDN with the Symplicity® system. Norepinephrine (NE) renal gradients were determined before and 1 month after RDN. Renal arteries were processed for histological (hematoxylin-eosin, Movat pentachrome) and immunohistochemical (S100, tyrosine-hydroxylase) analyses. BP significantly rose after DOCA implants. Six animals died prematurely, mainly from infectious causes. The surviving animals showed stable BP levels after 5 months. One month after RDN, nerve damage was showed in three animals, with impedance drop >10%, NE gradient drop and reduction in BP. The fourth animal showed no nerve damage, impedance drop <10%, NE gradient increase and no change in BP. In conclusion, the minipig model of DOCA-induced HTN is feasible, showing durable effects. High mortality should be addressed in next iterations of this model. RDN may partially offset the DOCA-induced HTN. Impedance drop and NE renal gradient could be markers of RDN success.
PB MDPI
LK https://hdl.handle.net/10612/18540
UL https://hdl.handle.net/10612/18540
NO de Prado, A. P., Pérez-Martínez, C., Regueiro-Purriños, M., Cuellas-Ramón, C., López-Benito, M., Gonzalo-Orden, J. M., Estévez-Loureiro, R., Cortina-Rivero, A. I., Viñuela-Baragaño, D., Altonaga, J. R., Tellez, A., & Fernández-Vázquez, F. (2020). Development and evaluation of a disease large animal model for preclinical assessment of renal denervation therapies. Animals, 10(9), 1-15. https://doi.org/10.3390/ANI10091446
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024