RT info:eu-repo/semantics/article
T1 Experimental Infection of Captive Red Foxes (Vulpes vulpes) with Mycobacterium bovis
A1 Richomme, Céline
A1 Lesellier, Sandrine
A1 Salguero, Francisco Javier
A1 Barrat, Jacques Laurent
A1 Boucher, Jean-Marc
A1 Reyes-Reyes, Jennifer Danaidae
A1 Hénault, Sylvie
A1 De Cruz, Krystel
A1 Tambosco, Jennifer
A1 Michelet, Lorraine
A1 Boutet, Justine
A1 Elahi, Rubyat
A1 Lyashchenko, Konstantin P.
A1 O’Halloran, Conor
A1 Balseiro Morales, Ana María
A1 Boschiroli, Maria Laura
A2 Sanidad Animal
K1 Sanidad animal
K1 Tuberculosis
K1 Red fox
K1 Vulpes vulpes
K1 Pathogenesis
K1 Excretion
K1 Serology
K1 3109 Ciencias Veterinarias
AB [EN] In Europe, animal tuberculosis (TB) due to Mycobacterium bovis involves multi-host communities that include cattle and wildlife species, such as wild boar (Sus scrofa), badgers (Meles meles) and red deer (Cervus elaphus). Red fox (Vulpes vulpes) infections have also been recently reported in some TB endemic regions in the Iberian Peninsula and France, with some of the infected animals shedding M. bovis in urine and feces. In order to understand the pathogenesis of M. bovis infection in foxes and the associated risk of transmission, 12 captive foxes (6 females and 6 males) were inoc-ulated orally with 2 × 107 colony-forming units of a French field isolate of M. bovis. Clinical samples (urine, feces and oropharyngeal swabs) were collected every four weeks and tested for molecular diagnosis and bacteriology. Serological responses were measured by IDEXX M. bovis Ab Test and Multi Antigen Print Immunoassay (MAPIA). At a post-mortem examination performed 12 weeks post infection (wpi), tissues were tested for the presence of M. bovis and associated gross and microscopic TB-like lesions. M. bovis was detected by PCR in bladder swabs of 3 animals at 12 wpi. It was also detected pre-mortem at different time points of the experiment in the oropharyngeal mu-cus of three individuals and in the feces of nine foxes, with two of them confirmed by bacteriology. All 12 foxes had at least 4 PCR positive samples (out of the 23 tested), and all but 1 fox had at least 1 culture positive sample. The culture negative fox was PCR positive in both retropharyngeal and mesenteric lymph nodes, in line with the results of the other animals. Seroconversion was observed in all foxes except one during the experiment, and in nine at the final time point. No gross visible lesions were found in any animal at the post-mortem examination. The histology showed small granulomas within the lymph nodes, tonsils, liver and lungs from eight animals, with the presence of few acid-fast bacilli. These results confirmed that all orally-infected foxes developed mild TB lesions but they were able to shed mycobacteria in about 75% of cases, 1 month post-infection (9 out 12 foxes). These results show that it is possible to induce typical TB infection experimentally in captive foxes, with measurable M. bovis excretion; such an experimental system could be useful for future evaluations of diagnostics and vaccines in this species
PB MDPI
LK https://hdl.handle.net/10612/18740
UL https://hdl.handle.net/10612/18740
NO Richomme, C., Lesellier, S., Salguero, F. J., Barrat, J. L., Boucher, J.-M., Reyes-Reyes, J. D., Hénault, S., De Cruz, K., Tambosco, J., Michelet, L., Boutet, J., Elahi, R., Lyashchenko, K. P., O’halloran, C., Balseiro, A., & Boschiroli, M. L. (2022). Experimental Infection of Captive Red Foxes (Vulpes vulpes) with Mycobacterium bovis. Microorganisms, 10(2). https://doi.org/10.3390/MICROORGANISMS10020380
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 02-jun-2024