RT info:eu-repo/semantics/article
T1 Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis
A1 Peña Cearra, Ainize
A1 Pascual Itoiz, Miguel Ángel
A1 Lavín, José Luis
A1 Fuertes Franco, Miguel
A1 Martín Ruiz, Itziar
A1 Castelo, Janire
A1 Palacios, Ainhoa
A1 Barriales, Diego
A1 Fullaondo, Asier
A1 Aransay, Ana M.
A1 Rodriguez, Héctor
A1 Anguita, Juan
A1 Abecia, Leticia
A2 Sanidad Animal
K1 Biología
K1 Inmunología
K1 Medicina. Salud
K1 Sanidad animal
K1 Immunology
K1 Microbiology
K1 2412 Inmunología
K1 3109.03 Inmunología
K1 2414 Microbiología
K1 3109.05 Microbiología
AB [EN]Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors. MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria. Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency. Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation. This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype. Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice. However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity. These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
PB Nature Research
LK https://hdl.handle.net/10612/18806
UL https://hdl.handle.net/10612/18806
NO Peña-Cearra, A., Pascual-Itoiz, M. A., Lavín, J. L., Fuertes, M., Martín-Ruiz, I., Castelo, J., Palacios, A., Barriales, D., Fullaondo, A., Aransay, A. M., Rodríguez, H., Anguita, J., & Abecia, L. (2022). Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis. Scientific Reports, 12(1). https://doi.org/10.1038/S41598-022-13480-Y
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 23-may-2024