RT info:eu-repo/semantics/article
T1 Effects of trichothecene production by Trichoderma arundinaceum isolates from bean-field soils on the defense response, growth and development of bean plants (Phaseolus vulgaris)
A1 Cardoza, Rosa E.
A1 Mayo Prieto, Sara
A1 Martínez Reyes, Natalia
A1 McCormick, Susan P.
A1 Carro-Huerga, Guzmán
A1 Campelo Rodríguez, María Piedad
A1 Rodríguez González, Álvaro
A1 Lorenzana de la Varga, Alicia
A1 Proctor, Robert Henry
A1 Casquero Luelmo, Pedro Antonio
A1 Gutiérrez Martín, Santiago
A2 Microbiologia
K1 Bioquímica
K1 Biological control
K1 Gene-clusters
K1 Metabolomics
K1 Plant-fungal interaction
K1 Secondary metabolites
K1 Sesquiterpenes
K1 Transcriptomics
AB [EN] The trichothecene toxin-producing fungus Trichoderma arundinaceum has potential as a biological control agent. However, most biocontrol studies have focused only on one strain, IBT 40837. In the current study, three Trichoderma isolates recovered from bean-field soils produced the trichothecene harzianum A (HA) and trichodermol, the latter being an intermediate in the HA biosynthesis. Based on phylogenetic analysis, the three isolates were assigned to the species T. arundinaceum. Their genome sequences had a high degree of similarity to the reference IBT 40837 strain, in terms of total genome size, number of predicted genes, and diversity of putative secondary metabolite biosynthetic gene clusters. HA production by these bean-field isolates conferred significant in vitro antifungal activity against Rhizoctonia solani and Sclerotinia sclerotiorum, which are some of the most important bean pathogens. Furthermore, the bean-field isolates stimulated germination of bean seeds and subsequent growth of above ground parts of the bean plant. Transcriptomic analysis of bean plants inoculated with these T. arundinaceum bean-field soil isolates indicated that HA production significantly affected expression of plant defense-related genes; this effect was particularly significant in the expression of chitinase-encoding genes. Together, these results indicate that Trichoderma species producing non-phytotoxic trichothecenes can induce defenses in plants without negatively affecting germination and development
PB Frontiers Media
LK https://hdl.handle.net/10612/18906
UL https://hdl.handle.net/10612/18906
NO Cardoza, R. E., Mayo-Prieto, S., Martínez-Reyes, N., McCormick, S. P., Carro-Huerga, G., Campelo, M. P., Rodríguez-González, Á., Lorenzana, A., Proctor, R. H., Casquero, P. A., & Gutiérrez, S. (2022). Effects of trichothecene production by Trichoderma arundinaceum isolates from bean-field soils on the defense response, growth and development of bean plants (Phaseolus vulgaris). Frontiers in Plant Science, 13. https://doi.org/10.3389/FPLS.2022.1005906
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 11-may-2024