RT info:eu-repo/semantics/article
T1 Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
A1 Rilo, Máximo Petrocchi
A1 Gutiérrez Martín, César Bernardo
A1 Pérez Fernández, Esther
A1 Vilaró, Anna
A1 Fraile, Lorenzo
A1 Martínez Martínez, Sonia
A2 Sanidad Animal
K1 Sanidad animal
K1 Pasteurella multocida
K1 Antimicrobial resistance genes
K1 Antimicrobial susceptibility patterns
K1 Swine
K1 3109 Ciencias Veterinarias
AB [EN] Forty‐eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in “Castilla y León” (north‐western Spain) in 2017–2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed
PB MDPI
LK https://hdl.handle.net/10612/18982
UL https://hdl.handle.net/10612/18982
NO Petrocchi‐rilo, M., Gutiérrez‐martín, C.-B., Pérez‐fernández, E., Vilaró, A., Fraile, L., & Martínez‐martínez, S. (2020). Antimicrobial resistance genes in porcine pasteurella multocida are not associated with its antimicrobial susceptibility pattern. Antibiotics, 9(9), 1-11. https://doi.org/10.3390/ANTIBIOTICS9090614
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024