RT info:eu-repo/semantics/article
T1 Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants
A1 González García, Sandra
A1 Álvarez Pérez, José Manuel
A1 Sáenz de Miera Carnicer, Luis Enrique
A1 Cobos Román, Rebeca
A1 Ibáñez Sánchez, Ana María
A1 Diez Galán, Alba
A1 Garzón Jimeno, José Enrique
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Bioquímica
K1 Biotecnología
K1 Streptomyces
K1 Grapevine
K1 Genomics
K1 Polymerase chain reaction
K1 Random amplified polymorphic DNA technique
K1 Rhizosphere
K1 DNA isolation
K1 Bacteria
K1 3108.02 Control Biológico de Enfermedades
K1 3108.05 Hongos
K1 2409.92 Genética Molecular de Plantas
K1 3108.01 Bacterias
K1 3108.08 Susceptibilidad y Resistencia Vegetal
AB [EN] The endophytic Streptomyces sp. VV/E1, and rhizosphere Streptomyces sp. VV/R4 strains, isolated from grapevine plants were shown in a previous work to reduce the infection rate of fungal pathogens involved in young grapevine decline. In this study we cloned fragments from randomly amplified polymorphic DNA (RAPD), and developed two stably diagnostic sequence-characterized amplified region (SCAR) markers of 182 and 160 bp for the VV/E1 and VV/R4 strains, respectively. The SCAR markers were not found in another 50 actinobacterial strains isolated from grapevine plants. Quantitative real-time PCR protocols based on the amplification of these SCAR markers were used for the detection and quantification of both strains in plant material. These strains were applied on young potted plants using two methods: perforation of the rootstock followed by injection of the microorganisms or soaking the root system in a bacterial suspension. Both methods were combined with a booster treatment by direct addition of a bacterial suspension to the soil near the root system. Analysis of uprooted plants showed that those inoculated by injection exhibited the highest rate of colonization. In contrast, direct addition of either strain to the soil did not lead to reliable colonization. This study has developed molecular tools for analyzing different methods for inoculating grapevine plants with selected Streptomyces sp. strains which protect them from fungal infections that enter through their root system. These tools are of great applied interest since they could easily be established in nurseries to produce grafted grapevine plants that are protected against fungal pathogens. Finally, this methodology might also be applied to other vascular plants for their colonization with beneficial biological control agents
PB Public Library of Science (PLoS)
LK https://hdl.handle.net/10612/19271
UL https://hdl.handle.net/10612/19271
NO González-García, S., Álvarez-Pérez, J. M., Sáenz de Miera, L. E., Cobos, R., Ibañez, A., Díez-Galán, A., Garzón-Jimeno, E., & Coque, J. J. R. (2019). Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. Strains into grapevine plants. PLoS ONE, 14(1), Article e0211225. https://doi.org/10.1371/journal.pone.0211225
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-may-2024