RT info:eu-repo/semantics/article
T1 Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium
A1 Elhadidy, Mohamed
A1 Miller, William G.
A1 Argüello Rodríguez, Héctor
A1 Álvarez Ordóñez, Avelino
A1 Duarte, Alexandra
A1 Dierick, Katelijne
A1 Botteldoorn, Nadine
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Broiler
K1 Antibiotic resistance
K1 Resistance genes
K1 Mutations
K1 MLST
K1 3109 Ciencias Veterinarias
K1 3109.05 Microbiología
K1 2414.01 Antibióticos
AB [EN]Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204 C. jejuni isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin. C. jejuni isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the gyrA gene, while C. jejuni isolates resistant to tetracycline were screened for the presence of the tet(O) gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the rplD and rplV ribosomal genes, and the intergenic region between cmeR and cmeABC. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene gyrA (p = 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in C. jejuni, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes.
PB Frontiers Media
SN 1664-302X
LK https://hdl.handle.net/10612/19438
UL https://hdl.handle.net/10612/19438
NO Elhadidy, M., Miller, W. G., Arguello, H., Álvarez-Ordóñez, A., Duarte, A., Dierick, K., & Botteldoorn, N. (2018). Genetic basis and clonal population structure of antibiotic resistance in Campylobacter jejuni isolated from broiler carcasses in Belgium. Frontiers in Microbiology, 9, Article e1014. https://doi.org/10.3389/FMICB.2018.01014Á
NO Copyright © 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 03-jun-2024