RT info:eu-repo/semantics/article
T1 Rapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasma
A1 Fernández, Nélida
A1 García, Juan J.
A1 Diez Liébana, María José
A1 Terán Somaza, María Teresa
A1 Sierra Vega, Matilde
A2 Farmacologia
K1 Ecología. Medio ambiente
K1 Farmacología
K1 Veterinaria
K1 Chromatographic
K1 Ethinylestradiol
K1 Rabbit plasma
AB A method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phasehigh-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of + 1 V vs. Ag/AgCI. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min , the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 mlof plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.
PB Elsevier
YR 2015
FD 2015-09-04
LK http://hdl.handle.net/10612/4574
UL http://hdl.handle.net/10612/4574
NO Journal of Chromatography, 1993, n. 619
NO P. 143-147
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 02-may-2024