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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorDomínguez Rebolledo, Álvaro Efrén
dc.contributor.authorFernández Santos, María Rocío
dc.contributor.authorBisbal Vigo, Alfonso
dc.contributor.authorRos Santaella, José Luis
dc.contributor.authorRamón Fernández, Manuel
dc.contributor.authorCarmona Delgado, Manuel
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.otherBiologia Celulares_ES
dc.date2010-04-15
dc.date.accessioned2019-04-14T23:24:06Z
dc.date.available2019-04-14T23:24:06Z
dc.date.issued2019-04-15
dc.identifier.citationReproduction, Fertility and Development, 2010, vol. 22, n. 5es_ES
dc.identifier.otherhttp://www.publish.csiro.au/rd/rd09197es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10222
dc.descriptionP. 856-870es_ES
dc.description.abstractAntioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability of lipoic acid, melatonin, Trolox and crocin was tested on red deer spermatozoa incubated at 37°C. Cryopreserved spermatozoa were thawed and incubated with 1 mM or 0.1 mM of each antioxidant, with or without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential and acrosomal status were assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were checked at 4 h. Incubation alone increased ROS and decreased motility. Oxidative stress intensified these effects, increasing lipoperoxidation and DNA damage. Lipoic acid had little protective effect, whereas 1 mM melatonin showed limited protection. Trolox lowered ROS and lipoperoxidation both in oxidised and non-oxidised samples. In oxidised samples, Trolox prevented DNA and acrosomal damage, and ameliorated motility. Crocin at 1 mM showed similar results to Trolox, but noticeably stimulated motility and had no effect on lipoperoxidation. In a second experiment, a broader range of crocin and melatonin concentrations were tested, confirming the effects of crocin (positive effects noticeable at 0.5–0.75 mM), but showing an increase in lipoperoxidation at 2 mM. Melatonin was increasingly effective at 2.5 and 5 mM (ROS, lipoperoxidation and DNA status). Crocin seems a promising new antioxidant, but its particular effects on sperm physiology must be further studied, especially the consequences of motility stimulation and confirming its effect on lipoperoxidation. Melatonin might be useful at relatively high concentrations, compared to Trolox.es_ES
dc.languageenges_ES
dc.publisherCSIROes_ES
dc.subjectVeterinariaes_ES
dc.subject.otherCrocines_ES
dc.subject.otherLipoic acides_ES
dc.subject.otherMelatonines_ES
dc.subject.otherTroloxes_ES
dc.titleImproving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatmentses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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