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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorAnel López, Luis 
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorGarcía Álvarez, Olga
dc.contributor.authorÁlvarez García, Mercedes 
dc.contributor.authorMaroto Morales, Alejandro
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.authorPaz Cabello, Paulino de 
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2012-11
dc.date.accessioned2019-04-25T13:49:34Z
dc.date.available2019-04-25T13:49:34Z
dc.date.issued2019-04-25
dc.identifier.citationAnimal Reproduction Science, 2012, vol. 135, n. 1–4es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0378432012002758?via%3Dihubes_ES
dc.identifier.urihttp://hdl.handle.net/10612/10658
dc.descriptionP. 37-46es_ES
dc.description.abstractThe use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the antioxidants Trolox and reduced glutathione (GSH) for freezing red deer epididymal spermatozoa, aiming at improving post-thawing quality. Samples from 19 stags were frozen in a TES-Tris-fructose extender (20% egg yolk, 8% glycerol), with 1 or 5 mM of antioxidant. Motility (CASA), lipoperoxidation (malondialdehyde –MDA– production), membrane status, mitochondrial activity, acrosomal status (flow cytometry) and chromatin status (SCSA: %DFI and %HDS; flow cytometry) were assessed after thawing and after 6 h at 39 °C. There were few differences between treatments after thawing, with Trolox reducing MDA production in a dose–response manner. After the incubation, sperm quality decreased and %DFI increased moderately, with no change for MDA. GSH improved motility, kinematic parameters and mitochondrial status, with a slight increase in %HDS. GSH 5 mM also increased moderately MDA production and %DFI, possibly due to enhanced metabolic activity and reducing power. Trolox maintained MDA low, but was detrimental to sperm quality. Trolox might not be appropriate for the cryopreservation of red deer epididymal spermatozoa, at least at the millimolar range. GSH results are promising, especially regarding motility improvement after the post-thawing incubation, and should be selected for future fertility trials.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherEpididymal spermes_ES
dc.subject.otherRed deeres_ES
dc.subject.otherAntioxidantes_ES
dc.subject.otherTroloxes_ES
dc.subject.otherGSHes_ES
dc.subject.otherCryopreservationes_ES
dc.titleReduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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