dc.description.abstract | Liposomes could improve the delivery
of substances to sperm. This study was conducted to
investigate the effect of the antioxidant ellagic acid
and ellagic acid-loaded liposomes on post-thawed sperm
quality in broiler breeder roosters. Semen was diluted
in Beltsville extender containing ellagic acid or ellagic
acid-loaded liposomes (ellagic acid at 0 (control),
0.5, 1, and 2 mM) and cryopreserved. Sperm quality
was evaluated post-thawing: motility characteristics
(Computer-Assisted Semen Analysis), membrane
functionality (HOS test), abnormal morphology, mitochondrial
activity (Rhodamine 123), apoptotic status
(Annexin V/Propidium iodide), malondialdehyde, and
antioxidant activities (glutathione peroxidase (GPx),
superoxide dismutase (SOD), and total antioxidant
capacity (TAC)). The results showed that 1 mM ellagic
acid-loaded liposomes improved total motility, membrane
functionality, and viability comparing to 0.5 and
2 mM ellagic acid, 2 mM ellagic acid-loaded liposomes,
and control group. Mitochondrial activity was significantly
higher for 1 mM ellagic acid-loaded liposomes
compared to the rest of the treatments, except 1 mM
ellagic acid. Ellagic acid at 1 mM in both forms significantly
increased GPx and TAC after freeze-thawing
(no significant variation for SOD), and also yielded the
lower proportion of apoptotic and dead cells. In conclusion,
ellagic acid improved post-thawed sperm quality
in broiler breeder roosters. The use of liposomes could
further enhance the effects of ellagic acid. | es_ES |