Mostrar el registro sencillo del ítem

dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorÁlvarez García, Mercedes 
dc.contributor.authorBarragán Santos, Santiago
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorHolt, William Vincent
dc.contributor.authorFazeli, Alireza
dc.contributor.authorPaz Cabello, Paulino de 
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.otherBiologia Celulares_ES
dc.date2013-02
dc.date.accessioned2019-05-17T13:15:11Z
dc.date.available2019-05-17T13:15:11Z
dc.date.issued2019-05-17
dc.identifier.citationTheriogenology, 2013, vol. 79, n. 3es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X12006024#!es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10780
dc.descriptionP. 541-550es_ES
dc.description.abstractThe Cantabrian brown bear survives as a small remnant population in northern Spain and semen cryopreservation for future artificial insemination is one of the measures being implemented for conservation of this species. As part of this program we investigated the value of adding heat shock protein A8 (HSPA8) to media (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid-TRIS-fructose with 20% egg yolk) used for chilling and cryopreserving the spermatozoa. Semen samples from eight brown bears were obtained by electroejaculation during the breeding season. In experiment 1, we tested three concentrations of HSPA8 (0.5, 1, and 5 μg/mL) to determine whether sperm motility (computer assisted sperm analysis system) and sperm survival could be improved during refrigeration (5 °C) up to 48 hours. Results showed that sperm viability (test with propidium iodide) was improved by the addition of 0.5 and 5 μg/mL HSPA8. In experiment 2, HSPA8 was added to the cryopreservation media (6% final glycerol concentration) before the freezing process. Though there were no differences in sperm viability immediately after thawing (analyses to 0 hours), plasma membrane permeability (test with YO-PRO-1) was significantly lower by the presence of HSPA8 (1 μg/mL) and acrosomal damage (test with peanut agglutinin-fluorescein isothiocyanate conjugate) was reduced by higher concentrations of HSPA8 (1 and 5 μg/mL) (analyses after thermal stress test incubating over 2 hours to 37 °C). In experiment 3, results of a simple progression test carried out through artificial mucus (hyaluronic acid 4 mg/mL) showed a significant decrease in the total number of sperm able to swim a distance of 0.5 to 2 cm through a capillary tube for all HSPA8-based extenders. Nevertheless, the distance traveled by the vanguard spermatozoa, which represent a highly motile subpopulation, was restored by the inclusion of 1 and 5 μg/mL HSPA8 in the cryopreservation media. Thus, the HSPA8 addition to extender improves the quality of brown bear (Ursus arctos) sperm during chilling (viability) and after cryopreservation (number of sperm with damaged acrosomes and “apoptotic-like” changes)es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherBrown beares_ES
dc.subject.otherSperm cryopreservationes_ES
dc.subject.otherHeat shock proteinses_ES
dc.subject.otherArtificial mucuses_ES
dc.titleThe addition of heat shock protein HSPA8 to cryoprotective media improves the survival of brown bear (Ursus arctos) spermatozoa during chilling and after cryopreservationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem