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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorFernández Santos, María Rocío
dc.contributor.authorDomínguez Rebolledo, Álvaro Efrén
dc.contributor.authorEsteso, Milagros
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2009-08
dc.date.accessioned2019-05-24T08:46:55Z
dc.date.available2019-05-24T08:46:55Z
dc.date.issued2019-05-24
dc.identifier.citationInternational Journal of Andrology, 2009, vol. 32, n. 4es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10824
dc.descriptionP. 353-359es_ES
dc.description.abstractThe potential protective effect of catalase supplementation during in vitro culture of frozen/thawed bull spermatozoa was investigated. Frozen/thawed semen collected from three fighting bulls was diluted in phosphate buffered saline (PBS) and incubated at 37 °C under different experimental conditions: Control, Catalase (CAT) (200 U/mL), Oxidant (OXI) (100 μm Fe2+/1 mm ascorbate), and Catalase + Oxidant (CAT/OXI). We assessed sperm motility, acrosomal integrity, viability and chromatin status (SCSA®) at 0, 2 and 6 h of incubation. Our results showed that catalase abolished the effect of the oxidant, protecting spermatozoa against reactive oxygen species, and improving both sperm motility and chromatin status during incubation. The OXI treatment significantly reduced the percentage of motile sperm after 6 h of incubation. The statistical model also showed that there were differences in sperm motility between CAT/OXI (20.8 ± 2.9%) and OXI (11.6 ± 7.6%) (p < 0.001). There were no significant effects of OXI on sperm viability, acrosomal status or proportion of abnormal tails. %DFI (spermatozoa with moderate or high DNA Fragmentation Index) was significantly higher on OXI (p < 0.001). Catalase prevented DNA fragmentation even in the presence of the oxidant (%DFI: 30.3 ± 0.8% OXI vs. 17.4 ± 0.7% CAT/OXI). We conclude that catalase supplementation after thawing could protect bull spermatozoa against oxidative stress, and it could improve media used for processing thawed spermatozoa.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherBulles_ES
dc.subject.otherCatalasees_ES
dc.subject.otherOxidative stresses_ES
dc.subject.otherSperm Chromatin Structure Assayes_ES
dc.subject.otherSperm chromatines_ES
dc.titleCatalase supplementation on thawed bull spermatozoa abolishes the detrimental effect of oxidative stress on motility and DNA integrityes_ES
dc.typeinfo:eu-repo/semantics/preprintes_ES


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