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Título
Molecular Diversity of ESBL-Producing Escherichia coli from Foods of Animal Origin and Human Patients
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
International Journal of Environmental Research and Public Health
Número de la revista
4
Editor
MDPI
Fecha
2020-02
Abstract
Abstract: Dissemination of enterobacteria that produce extended spectrum -lactamases (ESBL)
throughout the food chain has become an important health concern. This work aimed to evaluate the
occurrence of ESBL-producing bacteria in foods of animal origin and to investigate the similarities
between food and human isolates. The presence of beta-lactam-resistant Enterobacteriaceae was
analyzed in 108 food samples, isolating 10 strains of Escherichia coli, one strain of Citrobacter freundi,
and one of Hafnia alvei. E. coli isolates were compared to a group of 15 strains isolated from human
patients by antibiotic susceptibility testing, characterization of ESBL genes (blaTEM, blaCTX,), multilocus
sequence typing (MLST) and pulse-field gel electrophoresis (PFGE). Nineteen (14 clinical and five
food) isolates carried blaCTX, 14 (six clinical and eight food) carried blaTEM, and three (one clinical and
two food) carried blaSHV gen. MLST analysis revealed the prevalence of ST131 among the clinical
strains, which grouped together in a PFGE cluster. Food isolates showed higher diversity and two
of them (ST57) grouped with clinical strains, whereas another two belonged to clonal groups with
virulence potential (ST59). In conclusion, the results showed that foods of animal origin must be
regarded as a reservoir of ESBL-producing bacteria of clinical relevance, which might spread through
the food chain.
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