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Título
Novel two-component systems implied in antibiotic production in Streptomyces coelicolor
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
PLoS ONE
Número de la revista
5
Cita Bibliográfica
Yepes, A., Rico, S., Rodríguez-García, A., Santamaría, R. I., & Díaz, M. (2011). Novel two-component systems implied in antibiotic production in Streptomyces coelicolor. PloS one, 6(5), Article e19980. https://doi.org/10.1371/journal.pone.0019980
Editorial
Public Library of Science
Fecha
2011-05-20
Resumen
[EN] The abundance of two-component systems (TCSs) in Streptomyces coelicolor A3(2) genome indicates their importance in the physiology of this soil bacteria. Currently, several TCSs have been related to antibiotic regulation, and the purpose in this study was the characterization of five TCSs, selected by sequence homology with the well-known absA1A2 system, that could also be associated with this important process. Null mutants of the five TCSs were obtained and two mutants (ΔSCO1744/1745 and ΔSCO4596/4597/4598) showed significant differences in both antibiotic production and morphological differentiation, and have been renamed as abr (antibiotic regulator). No detectable changes in antibiotic production were found in the mutants in the systems that include the ORFs SCO3638/3639, SCO3640/3641 and SCO2165/2166 in any of the culture conditions assayed. The system SCO1744/1745 (AbrA1/A2) was involved in negative regulation of antibiotic production, and acted also as a negative regulator of the morphological differentiation. By contrast, the system SCO4596/4597/4598 (AbrC1/C2/C3), composed of two histidine kinases and one response regulator, had positive effects on both morphological development and antibiotic production. Microarray analyses of the ΔabrC1/C2/C3 and wild-type transcriptomes revealed downregulation of actII-ORF4 and cdaR genes, the actinorhodin and calcium-dependent antibiotic pathway-specific regulators respectively. These results demonstrated the involvement of these new two-component systems in antibiotic production and morphological differentiation by different approaches. One is a pleiotropic negative regulator: abrA1/A2. The other one is a positive regulator composed of three elements, two histidine kinases and one response regulator: abrC1/C2/C3
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MICYT//GEN2003-20245-C09-02
- info:eu-repo/grantAgreement/MICINN/ Programa Nacional de Investigación Fundamental/ BFU2010-17551/ES/CONTROL DE LA PRODUCCION DE ANTIBIOTICOS POR UN SISTEMA DE DOS COMPONENTES ATIPICO MUY CONSERVADO EN EL GENERO STREPTOMYCES
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