Compartir
Título
Development and Evaluation of a Disease Large Animal Model for Preclinical Assessment of Renal Denervation Therapies
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Animals
Número de la revista
9
Cita Bibliográfica
de Prado, A. P., Pérez-Martínez, C., Regueiro-Purriños, M., Cuellas-Ramón, C., López-Benito, M., Gonzalo-Orden, J. M., Estévez-Loureiro, R., Cortina-Rivero, A. I., Viñuela-Baragaño, D., Altonaga, J. R., Tellez, A., & Fernández-Vázquez, F. (2020). Development and evaluation of a disease large animal model for preclinical assessment of renal denervation therapies. Animals, 10(9), 1-15. https://doi.org/10.3390/ANI10091446
Editorial
MDPI
Fecha
2020
Resumen
[EN] New-generation catheters-based renal denervation (RDN) is under investigation for the treatment of uncontrolled hypertension (HTN). We assessed the feasibility of a large animal model of HTN to accommodate the human RDN devices. Ten minipigs were instrumented to measure blood pressure (BP) in an awake-state. HTN was induced with subcutaneous 11-deoxycorticosterone (DOCA, 100 mg/kg) implants. Five months after, the surviving animals underwent RDN with the Symplicity® system. Norepinephrine (NE) renal gradients were determined before and 1 month after RDN. Renal arteries were processed for histological (hematoxylin-eosin, Movat pentachrome) and immunohistochemical (S100, tyrosine-hydroxylase) analyses. BP significantly rose after DOCA implants. Six animals died prematurely, mainly from infectious causes. The surviving animals showed stable BP levels after 5 months. One month after RDN, nerve damage was showed in three animals, with impedance drop >10%, NE gradient drop and reduction in BP. The fourth animal showed no nerve damage, impedance drop <10%, NE gradient increase and no change in BP. In conclusion, the minipig model of DOCA-induced HTN is feasible, showing durable effects. High mortality should be addressed in next iterations of this model. RDN may partially offset the DOCA-induced HTN. Impedance drop and NE renal gradient could be markers of RDN success.
Materia
Palabras clave
Peer review
SI
URI
DOI
Versión del editor
Aparece en las colecciones
- Artículos [4641]
Ficheros en el ítem
Tamaño:
3.469
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF