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Título
Identification of Regulatory Functions of LncRNAs Associated With T. circumcincta Infection in Adult Sheep
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Frontiers in Genetics
Cita Bibliográfica
Chitneedi, P. K., Weikard, R., Arranz, J. J., Martínez-Valladares, M., Kuehn, C., & Gutiérrez-Gil, B. (2021). Identification of Regulatory Functions of LncRNAs Associated With T. circumcincta Infection in Adult Sheep. Frontiers in Genetics, 12. https://doi.org/10.3389/FGENE.2021.685341
Editorial
Frontiers media
Fecha
2021
Resumen
[EN] Several recent studies have demonstrated the role of long non-coding RNAs (lncRNAs) in regulating the defense mechanism against parasite infections, but no studies are available that investigated their relevance for immune response to nematode infection in sheep. Thus, the aim of the current study was to (i) detect putative lncRNAs that are expressed in the abomasal lymph node of adult sheep after an experimental infection with the gastrointestinal nematode (GIN) Teladorsagia circumcincta and (ii) to elucidate their potential functional role associated with the differential host immune response. We hypothesized that putative lncRNAs differentially expressed (DE) between samples from animals that differ in resistance to infection may play a significant regulatory role in response to nematode infection in adult sheep. To obtain further support for our hypothesis, we performed co-expression and functional gene enrichment analyses with the differentially expressed lncRNAs (DE lncRNAs). In a conservative approach, we included for this predictive analysis only those lncRNAs that are confirmed and supported by documentation of expression in gastrointestinal tissues in the current sheep gene atlas. We identified 9,105 putative lncRNA transcripts corresponding to 7,124 gene loci. Of these, 457 were differentially expressed lncRNA loci (DELs) with 683 lncRNA transcripts. Based on a gene co-expression analysis via weighted gene co-expression network analysis, 12 gene network modules (GNMs) were found significantly correlated with at least one of 10 selected target DE lncRNAs. Based on the principle of “guilt-by-association,” the DE genes from each of the three most significantly correlated GNMs were subjected to a gene enrichment analysis. The significant pathways associated with DE lncRNAs included ERK5 Signaling, SAPK/JNK Signaling, RhoGDI Signaling, EIF2 Signaling, Regulation of eIF4 and p70S6K Signaling and Oxidative Phosphorylation pathways. They belong to signaling pathway categories like Cellular Growth, Proliferation and Development, Cellular Stress and Injury, Intracellular and Second Messenger Signaling and Apoptosis. Overall, this lncRNA study conducted in adult sheep after GIN infection provided first insights into the potential functional role of lncRNAs in the differential host response to nematode infection
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ID proyecto
- info:eu-repo/grantAgreement/JCL/ /LE248U14/ES/ ANÁLISIS DEL TRANSCRIPTOMA DE LA MUCOSA Y LOS GANGLIOS ABOMASALES PARA LA IDENTIFICACIÓN DE GENES INVOLUCRADOS EN LA RESISTENCIA A LOS NEMATODOS GASTROINTESTINALES EN OVEJAS ADULTAS//
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-093535-B-I00/ES/ ANALISIS DEL PAPEL DE LAS DE MARCAS EPIGENETICAS Y DE LA VARIABILIDAD GENOMICA EN LA REGULACION DE LA TRANSCRIPCION EN TEJIDOS DE INTERES PRODUCTIVO EN EL GANADO OVINO LECHERO//
- info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ RYC-2015-18368/ES/ RYC-2015-18368//
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