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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Pires Espíndola, Julia | |
dc.contributor.author | Balbinott, Natalia | |
dc.contributor.author | Gressler, Letícia Trevisan | |
dc.contributor.author | Machado, Gustavo | |
dc.contributor.author | Klein, Catia Silene | |
dc.contributor.author | Rebelatto, Raquel | |
dc.contributor.author | Gutiérrez Martín, César Bernardo | |
dc.contributor.author | Kreutz, Luiz Carlos | |
dc.contributor.author | Schryvers, Anthony Bernard | |
dc.contributor.author | Frandoloso, Rafael | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2019 | |
dc.date.accessioned | 2024-04-03T09:12:02Z | |
dc.date.available | 2024-04-03T09:12:02Z | |
dc.identifier.citation | Espíndola, J. P., Balbinott, N., Gressler, L. T., Machado, G., Klein, C. S., Rebelatto, R., Martín, C. B. G., Kreutz, L. C., Schryvers, A. B., & Frandoloso, R. (2019). Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil. PeerJ, 7, Article e6817. https://doi.org/10.7717/PEERJ.6817 | es_ES |
dc.identifier.other | https://peerj.com/articles/6817/ | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19340 | |
dc.description.abstract | [EN]Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-Typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. | es_ES |
dc.language | eng | es_ES |
dc.publisher | PeerJ | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Disease mapping | es_ES |
dc.subject.other | Haemophilus parasuis | es_ES |
dc.subject.other | Typification | es_ES |
dc.subject.other | Serovars | es_ES |
dc.subject.other | Vaccines | es_ES |
dc.subject.other | Brazil | es_ES |
dc.title | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.7717/PEERJ.6817 | |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2167-8359 | |
dc.journal.title | PeerJ | es_ES |
dc.volume.number | 7 | es_ES |
dc.page.initial | e6817 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3109.05 Microbiología | es_ES |
dc.description.project | This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant 485807/2013-0) and by the Secretaria de Desenvolvimento Econômico Ciência e Tecnologia do Rio Grande do Sul (SDECT, grant 328-2500/14-0). Julia Pires Espíndola and Letícia Trevisan Gressler were supported by Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) Master and Postdoctoral fellowship, respectively. Natalia Balbinott was supported by a CNPq undergraduate fellowship. Luiz Carlos Kreutz has a CNPq PQ fellowship (307900/2016-9). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
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