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Título
Integrated analyses of the methylome and transcriptome to unravel sex differences in the perirenal fat from suckling lambs
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Frontiers in Genetics
Cita Bibliográfica
Fonseca, P. A. S., Alonso García, M., Pelayo, R., Marina, H., Esteban Blanco, C., Mateo, J., Gutiérrez Gil, B., Arranz, J.-J., & Suárez-Vega, A. (2022). Integrated analyses of the methylome and transcriptome to unravel sex differences in the perirenal fat from suckling lambs. Frontiers in Genetics, 13. https://doi.org/10.3389/FGENE.2022.1035063
Editorial
Frontiers Media
Fecha
2022
Resumen
[EN] In sheep, differences were observed regarding fat accumulation and fatty acid (FA) composition between males and females, which may impact the quality and organoleptic characteristics of the meat. The integration of different omics technologies is a relevant approach for investigating biological and genetic mechanisms associated with complex traits. Here, the perirenal tissue of six male and six female Assaf suckling lambs was evaluated using RNA sequencing and whole-genome bisulfite sequencing (WGBS). A multiomic discriminant analysis using multiblock (s)PLS-DA allowed the identification of 314 genes and 627 differentially methylated regions (within these genes), which perfectly discriminate between males and females. These candidate genes overlapped with previously reported QTLs for carcass fat volume and percentage of different FAs in milk and meat from sheep. Additionally, differentially coexpressed (DcoExp) modules of genes between males (nine) and females (three) were identified that harbour 22 of these selected genes. Interestingly, these DcoExp were significantly correlated with fat percentage in different deposits (renal, pelvic, subcutaneous and intramuscular) and were associated with relevant biological processes for adipogenesis, adipocyte differentiation, fat volume and FA composition. Consequently, these genes may potentially impact adiposity and meat quality traits in a sex-specific manner, such as juiciness, tenderness and flavour
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Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-093535-B-I00/ES/ ANALISIS DEL PAPEL DE LAS DE MARCAS EPIGENETICAS Y DE LA VARIABILIDAD GENOMICA EN LA REGULACION DE LA TRANSCRIPCION EN TEJIDOS DE INTERES PRODUCTIVO EN EL GANADO OVINO LECHERO//
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