Compartir
Título
Arabidopsis immune responses triggered by cellulose‐ and mixed‐linked glucan‐derived oligosaccharides require a group ofleucine‐rich repeat malectinreceptor kinases
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
The Plant Journal
Número de la revista
4
Cita Bibliográfica
Martín-Dacal, M., Fernández-Calvo, P., Jiménez-Sandoval, P., López, G., Garrido-Arandía, M., Rebaque, D., del Hierro, I., Berlanga, D. J., Torres, M. Á., Kumar, V., Mélida, H., Pacios, L. F., Santiago, J., & Molina, A. (2023). Arabidopsis immune responses triggered by cellulose- and mixed-linked glucan-derived oligosaccharides require a group of leucine-rich repeat malectin receptor kinases. Plant Journal, 113(4), 833-850. https://doi.org/10.1111/TPJ.16088
Editorial
Wiley-Blackwell
Fecha
2023
ISSN
0960-7412
Resumen
[EN] The plant immune system perceives a diversity of carbohydrate ligands from plant and microbial cell walls
through the extracellular ectodomains (ECDs) of pattern recognition receptors (PRRs), which activate
pattern-triggered immunity (PTI). Among these ligands are oligosaccharides derived from mixed-linked b-
1,3/b-1,4-glucans (MLGs; e.g. b-1,4-D-(Glc)2-b-1,3-D-Glc, MLG43) and cellulose (e.g. b-1,4-D-(Glc)3, CEL3). The
mechanisms behind carbohydrate perception in plants are poorly characterized except for fungal chitin
oligosaccharides (e.g. b-1,4-D-(GlcNAc)6, CHI6), which involve several receptor kinase proteins (RKs) with
LysM-ECDs. Here, we describe the isolation and characterization of Arabidopsis thaliana mutants impaired
in glycan perception (igp) that are defective in PTI activation mediated by MLG43 and CEL3, but not by
CHI6. igp1–igp4 are altered in three RKs – AT1G56145 (IGP1), AT1G56130 (IGP2/IGP3) and AT1G56140 (IGP4)
– with leucine-rich-repeat (LRR) and malectin (MAL) domains in their ECDs. igp1 harbors point mutation
E906K and igp2 and igp3 harbor point mutation G773E in their kinase domains, whereas igp4 is a T-DNA
insertional loss-of-function mutant. Notably, isothermal titration calorimetry (ITC) assays with purified ECDRKs
of IGP1 and IGP3 showed that IGP1 binds with high affinity to CEL3 (with dissociation constant
KD = 1.19 0.03 lM) and cellopentaose (KD = 1.40 0.01 lM), but not to MLG43, supporting its function as
a plant PRR for cellulose-derived oligosaccharides. Our data suggest that these LRR-MAL RKs are components
of a recognition mechanism for both cellulose- and MLG-derived oligosaccharide perception and
downstream PTI activation in Arabidopsis.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/ RTI2018-096975-B-I00/ES/ RESPUESTAS DE INMUNIDAD VEGETAL REGULADAS POR PATRONES MOLECULARES ASOCIADOS A DAÑO: DEL RECONOCIMIENTO DE DAMPS (DAMAGE-ASSOCIATED MOLECULAR PATTERNS) A LA RESISTENCIA
URI
DOI
Aparece en las colecciones
- Artículos [5241]
Ficheros en el ítem
Tamaño:
4.540
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF