dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Vizcaíno, Juan Antonio | |
dc.contributor.author | Sanz Andreu, Luis | |
dc.contributor.author | Cardoza, Rosa E. | |
dc.contributor.author | Monte Vázquez, Enrique | |
dc.contributor.author | Gutiérrez Martín, Santiago | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2005 | |
dc.date.accessioned | 2024-07-01T08:25:52Z | |
dc.date.available | 2024-07-01T08:25:52Z | |
dc.identifier.citation | Vizcaíno, Sanz, Cardoza, Monte, & Gutiérrez. (2005). Detection of putative peptide synthetase genes in Trichoderma species: Application of this method to the cloning of a gene from T. harzianum CECT 2413. FEMS Microbiology Letters, 244(1), 139-148. https://doi.org/10.1016/J.FEMSLE.2005.01.036 | es_ES |
dc.identifier.issn | 0378-1097 | |
dc.identifier.other | https://academic.oup.com/femsle/article/244/1/139/546096?login=true | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/21684 | |
dc.description.abstract | [EN] Some of the secondary metabolites produced by Trichoderma, such as the peptaibols and other antibiotics, have a peptide structure and in their biosynthesis are involved proteins belonging to the Non-Ribosomal Peptide Synthetase family. In the present work, a PCR-mediated strategy was used to clone a region corresponding to an adenylation domain of a peptide synthetase (PS) gene from 10 different strains of Trichoderma. In addition, and using the fragment isolated by PCR from T. harzianum CECT 2413 as a probe, a fragment of 19.0 kb corresponding to a PS-encoding gene named salps1, including a 1.5 kb fragment of the promoter, was cloned and sequenced. The cloned region of salps1 contains four complete, and a fifth incomplete, modules, in which are found the adenylation, thiolation and condensation domains, but also an additional epimerization domain at the C-terminal end of the first module. The analysis of the Salps1 protein sequence, taking into consideration published data, suggests that it is neither a peptaibol synthetase nor a protein involved in siderophore biosynthesis. The presence of two breaks in the open reading frame and the expression of this gene under nitrogen starvation conditions suggest that salps1 could be a pseudogene. © 2005 Federation of European Microbiological Societies | es_ES |
dc.language | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | Attribution-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/4.0/ | * |
dc.subject | Biología | es_ES |
dc.subject.other | Trichoderma | es_ES |
dc.subject.other | Non-ribosomal peptide synthetase | es_ES |
dc.subject.other | Degenerated-PCR | es_ES |
dc.subject.other | Antibiosis | es_ES |
dc.subject.other | Pseudogene | es_ES |
dc.title | Detection of putative peptide synthetase genes inTrichodermaspecies: Application of this method to the cloning of a gene fromT. harzianumCECT 2413 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1016/J.FEMSLE.2005.01.036 | |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1574-6968 | |
dc.journal.title | FEMS Microbiology Letters | es_ES |
dc.volume.number | 244 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 139 | es_ES |
dc.page.final | 148 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.project | The authors acknowledge the financial support of the Spanish Foundations ‘‘Ramo´n Areces’’ (Madrid, Spain) and ‘‘Fundacio´n Andaluza de I+D’’ (Seville, Spain). This research was also supported by the European Commission (project QLK3-CT-2002-02032) | es_ES |