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Título
Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis
Autor
Facultad/Centro
Área de conocimiento
Datos de la obra
FEMS Immunology and Medical Microbiology, 2005
Editor
Elsevier
Fecha
2005-04-23
Zusammenfassung
Haemophilus parasuis is the causative agent of Glässer's disease, which is responsible for considerable economic losses in the pigrearing
industry. The aim of the study reported here was the identification, sequencing and molecular characterization of the TonB
region that includes tonB, exbBD, and tbpBA genes in H. parasuis. In addition, two fusion proteins were generated. One of them
(pGEX-6P-1-GST-TbpB) contained the first 501 amino acids of H. parasuis TbpB protein, while the second (pBAD-Thio-TbpBV5-
His) included the first 102 amino acids of H. parasuis TbpB N-terminus domain. A panel of 14 hybridomas secreting monoclonal
antibodies was raised against the two recombinant TbpB fusion proteins. Furthermore, to assess whether the expression of the
H. parasuis ExbB, TbpB, and TbpA proteins was upregulated under conditions of restricted availability of iron, a rabbit polyclonal
antibody against H. parasuis TbpB-His fusion protein was produced. A rabbit polyclonal antibody against serotype 7 of Actinobacillus
pleuropneumoniae ExbB and TbpA proteins was also used for the detection of the homologous proteins in H. parasuis. Overall,
the data indicate that H. parasuis, like other members of the Pasteurellaceae family, possesses the genetic elements of the TonB
region for iron acquisition and the transferrin-binding proteins encoded under this region are upregulated under restricted iron
availability
Materia
Palabras clave
Peer review
SI
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