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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorMartínez Martínez, Sonia 
dc.contributor.authorFrandoloso, Rafael
dc.contributor.authorRodríguez Ferri, Elías Fernando 
dc.contributor.authorGil García, María Concepción
dc.contributor.authorHernández Haro, Carolina
dc.contributor.authorYubero-Delgado, Sheila
dc.contributor.authorGutiérrez Martín, César Bernardo 
dc.contributor.otherSanidad Animales_ES
dc.date2012-11-26
dc.date.accessioned2017-10-19T11:06:46Z
dc.date.available2017-10-19T11:06:46Z
dc.date.issued2017-10-19
dc.identifier.citationVeterinary Immunology and Immunopathology, vol. XXXes_ES
dc.identifier.urihttp://hdl.handle.net/10612/6870
dc.description14 p.es_ES
dc.description.abstractAn immunoproteomic analysis of the protective response of subunit and commercial vaccines in colostrumdeprived pigs against Glässer’s disease was carried out. A mixture of proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki strain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp), along with a commercial bacterin. PAPT were separated using 2 dimensional electrophoresis (2DE) gels and with them, 2DE Western blots were carried out. A total of 17 spots were identified as positive with sera of pigs from any of the three vaccinated groups, the highest number of immunoreactive proteins being detected in those having received PAPTCp. Among them, six proteins (FKBP-type peptidyl-prolyl cis-trans isomerase, neuraminidase exo- -sialidase, xanthine-guanine phosphoribosyl transferase, CMP-N-acetylneuraminic acid synthetase, phenylalanyl-tRNA synthetase and glyceraldehyde 3-phosphate dehydrogenase) were found to be novel immunogens in H. parasuis. These proteins showed a high potential as candidates in future subunit vaccines against Glässer’s disease. The three experimental groups developed specific systemic total IgG (IgGt), IgG1, IgG2 and IgM antibodies after immunizations. In addition, those receiving PAPTCp yielded a serum IgA response.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectSanidad animales_ES
dc.subjectVeterinariaes_ES
dc.subject.otherGlässer’s diseasees_ES
dc.subject.otherHaemophilus parasuises_ES
dc.subject.otherAntibody responsees_ES
dc.subject.otherPiges_ES
dc.subject.otherImmunoproteomicses_ES
dc.titleImmunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer’s disease in pigses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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