RT info:eu-repo/semantics/article
T1 Evaluation of Three Different Extenders for Use in Emergency Salvaging of Epididymal Spermatozoa from a Cantabric Brown Bear
A1 Anel Rodríguez, Luis
A1 Álvarez García, Mercedes
A1 Anel Rodríguez, Enrique
A1 Martínez Pastor, Felipe
A1 Martínez, Félix
A1 Chamorro Álvarez, César Ángel
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Osos pardos
K1 Post mortem
K1 Criopreservación
K1 Esperma
K1 Epidídimo
K1 Extensores
AB The Cantabrian brown bear (Ursus arctos) constitutes an endangered subpopulation of the European brown bear in the north of Spain. We have carried out a post‐mortem recovery of epididymal spermatozoa from a Cantabrian brown bear (7 years old, 170 kg; 30 min post‐mortem), cryopreserving those recovered from the cauda epididymis (929 × 106 spermatozoa, 54% motile, 82% cytoplasmic droplets). For freezing, three extenders based on Test‐Tris‐Fructose + 4% glycerol were used: (1) 325 mOsm/kg and 10% egg yolk; (2) 430 mOsm/kg and 15% egg yolk; (3) 300 mOsm/kg, Equex‐EDTA and 20% egg yolk. After thawing, we obtained higher motility for extender 3 (31%), but extender 2 yielded the highest viability (66.9%) and mitochondrial activity (67.1%). Caffeine stimulation showed that extender two rendered the highest recovery values of post‐thawing motility with respect to the fresh sample. In conclusion, epididymal spermatozoa of brown bear can be frozen applying an extender with osmolality similar to epididymal environment.
PB John Wiley & Sons
YR 2019
FD 2019-04-15
LK http://hdl.handle.net/10612/10224
UL http://hdl.handle.net/10612/10224
NO Reproduction in Domestic Animals, 2011, vol. 46, n. 1
NO P. e85-e90
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 06-may-2024