RT info:eu-repo/semantics/article
T1 Sperm Subpopulations in Iberian Red Deer Epididymal Sperm and Their Changes Through the Cryopreservation Process
A1 Martínez Pastor, Felipe
A1 García Macías, Vanesa
A1 Álvarez García, Mercedes
A1 Herráez Ortega, María Paz
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 CASA
K1 Cluster analysis
K1 Cryopreservation
K1 Epididymal sperm
K1 Epididymis
K1 Gamete biology
K1 Iberian red deer
K1 Sperm
K1 Sperm motilityand transport
AB We have applied a statistical protocol based on principal component analysis, clustering methods, and discriminant analysis for the identification of sperm subpopulations in computer-assisted sperm analysis (CASA) data. Samples were obtained from the cauda epididymis of 11 Iberian red deer and cryopreserved following a standard protocol. Motility by CASA was analyzed just after sperm recovery, just before freezing, and after thawing, and eight motility descriptors for each individual spermatozoon were recorded. Sperm viability and acrosomal status were also assessed. Subpopulation analysis was performed in four sequential steps: principal component analysis using the eight motility descriptors; nonhierarchical clustering analysis (k-means) using the first two principal components; hierarchical clustering analysis (UPGMA); and selection of the final number of clusters. Three clusters were obtained for each motility analysis: slow and nonlinear; rapid and linear; and rapid, high ALH, nonlinear. We detected variations in the clusters between treatments (initial, prefreezing and postthawed). Indeed, motility increased and linearity decreased in the prefreezing analysis. A discriminant analysis isolated three descriptors that were used again in the same statistical analysis, giving four clusters that resembled the pattern found in the first classification. We also performed a clustering analysis of the males according to prefreezing/postthawed variation of total motility, viability, and acrosomal status. The proportion of the linear subpopulations in the prefreezing treatment, in both clustering analyses, correlated positively with postthawed viability recovery. Our results show that clustering analysis of CASA data gives useful and practical information that is not obtained by conventional sperm analysis.
PB Oxford University Press
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10316
UL http://hdl.handle.net/10612/10316
NO Biology of Reproduction, vol. 72, n.  2
NO P. 316–327
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 01-may-2024