RT info:eu-repo/semantics/article
T1 Sperm Cryopreservation in Brown Bear (Ursus arctos): Preliminary Aspects
A1 Anel Rodríguez, Luis
A1 Álvarez García, Mercedes
A1 Martínez Pastor, Felipe
A1 Gomes Alves, Susana Cláudia
A1 Nicolás, M.
A1 Mata Campuzano, María
A1 Martínez, Félix
A1 Barragán Santos, Santiago
A1 Anel Rodríguez, Enrique
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Esperma
K1 Criopreservación
K1 Osos pardos
K1 Ursus arctos
AB The development of sperm cryopreservation procedures in brown bear is the basis for establishing a specific genetic resource bank aimed at the preservation of a Cantabric brown bear population, which is seriously threatened. Several issues complicate the development of these cryopreservation procedures: lack of previous specific studies, a high incidence of urospermia and spermagglutination observed in bear ejaculates. Moreover, the availability of individuals for research from these threatened populations is problematic. In the case of the Cantabric brown bear, we have used males from other populations, but of the same species, as surrogates, to carry out a direct extrapolation of the results. Urospermia – Moreover, 70% of the ejaculates are urine contaminated and spermagglutination have a detrimental effect on post‐thawing cell quality recovery in this species. Considering the high value of these samples (autochthonous population with few individuals), a pre‐selection of the ejaculates is not a viable alternative. Preventive methods reducing the mentioned detrimental effects need to be developed. On the basis of previous data, we can suppose that bear spermatozoa resist freezing injuries well. Nevertheless, because of the scarcity of this information, it is necessary to conduct further research on bear semen freezing under field conditions. Epidydimal spermatozoa can be important for genetic resource banking of threatened populations and thus specific cryobiological protocols need to be assayed. To date, 168 brown bear ejaculates have been frozen by the ITRA‐ULE group at the University of León (Spain) in the development of methodologies for the preservation of brown bear sperm.
PB John Wiley & Sons
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10319
UL http://hdl.handle.net/10612/10319
NO Reproduction in Domestic Animals, 2008, vol. 43, n. 8
NO P. 9-17
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-abr-2024