RT info:eu-repo/semantics/article
T1 Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts
A1 Martínez Pastor, Felipe
A1 Cabrita, Elsa
A1 Soares, Florbela
A1 Anel Rodríguez, Luis
A1 Dinis, María Teresa
A2 Biologia Celular
K1 Veterinaria
K1 Lenguado senegalés
K1 Solea senegalensis
K1 Esperma
K1 CASA
K1 Teleósteos marinos
AB Computer-assisted sperm analysis (CASA) and clustering analysis have enabled to study sperm subpopulations in mammals, but their use in fish sperm has been limited. We have used spermatozoa from Senegalese sole (Solea senegalensis) as a model for subpopulation analysis in teleostei using two different activating solutions. Semen from six males was activated using 1100 mOsm/kg solutions: artificial seawater (ASW) or sucrose solution (SUC). Motility was acquired at 15, 30, 45, and 60 s post-activation. CASA parameters were combined into two principal components, which were used in a non-hierarchical clustering analysis, obtaining four subpopulations (CL): CL1 (slow/non-linear), CL2 (slow/linear), CL3 (fast/non-linear), and CL4 (fast/linear). We detected spermatozoa lysis, especially in ASW. Sperm motility was higher for SUC and decreased with time. The subpopulation proportions varied with time and activating treatment, showing both an increase in CL1 and CL2 and a decrease in CL3 and CL4 with time. Both CL3 and CL4 were higher in samples activated with SUC, at least in early post-activation. Proportions of CL3 and CL4 at 15 s were associated with higher quality at 60 s and with lower lysis. A second clustering analysis was conducted, classifying the males accordingly to their motility subpopulations. This analysis showed a high heterogeneity between samples. Subpopulation analysis of CASA data can be applied to Solea spermatozoa, allowing identification of potentially interesting sperm subpopulations. Future studies might benefit from these techniques to establish the relationship of these subpopulations with fish sperm quality and fertility, helping to characterize males according to their reproductive potential.
PB Bioscientifica
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10612/10608
UL http://hdl.handle.net/10612/10608
NO Reproduction, 2008, vol. 135, n. 4
NO P. 449–459
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-may-2024