RT info:eu-repo/semantics/article
T1 Response of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide, and Importance of Individual Male Variability
A1 Domínguez Rebolledo, Álvaro Efrén
A1 Martínez Pastor, Felipe
A1 Bisbal Vigo, Alfonso
A1 Ros Santaella, José Luis
A1 García Álvarez, Olga
A1 Maroto Morales, Alejandro
A1 Soler, Ana J.
A1 Garde López-Brea, Julián
A1 Fernández Santos, María Rocío
A2 Biologia Celular
K1 Veterinaria
K1 Ciervo rojo
K1 Esperma
K1 Agua
K1 Estrés oxidativo
AB Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H2O2) levels on red deer spermatozoa after cryopreservation, and the role of male‐to‐male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μm H2O2 for 2 h at 37°C. Intracellular reactive oxygen species (H2DCFDA‐CM) increased with H2O2 concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by sperm chromatin structure assay) with 200 μm. Lipoperoxidation assessed by the thiobarbituric acid reactive substance (TBARS) method increased slightly with 50 μm H2O2 and above. In a second experiment, samples from seven males were submitted to 0 and 200 μm H2O2 for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H2O2 presence. We found that the kinematic parameters reflected male‐to‐male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H2O2 or after incubation with H2O2. Red deer spermatozoa are relatively resilient to H2O2 after thawing, but it seems to be a great male‐to‐male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols.
PB John Wiley & Sons
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10612/10612
UL http://hdl.handle.net/10612/10612
NO Reproduction in Domestic Animals, 2011, vol. 46, n. 3
NO P. 393-403
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-abr-2024