RT info:eu-repo/semantics/article
T1 Seminal plasma applied post-thawing affects boar sperm physiology: A flow cytometry study
A1 Fernández Gago, Rocío
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Boar
K1 Semen
K1 Seminal plasma
K1 Sperm physiology
K1 Flow cytometry
AB Cryopreservation induces extensive biophysical and biochemical changes in the sperm. In the present study, we used flow cytometry to assess the capacitation-like status of frozen-thawed boar spermatozoa and its relationship with intracellular calcium, assessment of membrane fluidity, modification of thiol groups in plasma membrane proteins, reactive oxygen species (ROS) levels, viability, acrosomal status, and mitochondrial activity. This experiment was performed to verify the effect of adding seminal plasma on post-thaw sperm functions. To determine these effects after cryopreservation, frozen-thawed semen from seven boars was examined after supplementation with different concentrations of pooled seminal plasma (0%, 10%, and 50%) at various times of incubation from 0 to 4 hours. Incubation caused a decrease in membrane integrity and an increase in acrosomal damage, with small changes in other parameters (P > 0.05). Although 10% seminal plasma showed few differences with 0% (ROS increase at 4 hours, P < 0.05), 50% seminal plasma caused important changes. Membrane fluidity increased considerably from the beginning of the experiment, and ROS and free thiols in the cell surface increased by 2 hours of incubation. By the end of the experiment, viability decreased and acrosomal damage increased in the 50% seminal plasma samples. The addition of 50% of seminal plasma seems to modify the physiology of thawed boar spermatozoa, possibly through membrane changes and ROS increase. Although some effects were detrimental, the stimulatory effect of 50% seminal plasma could favor the performance of post-thawed boar semen, as showed in the field (García JC, Domínguez JC, Peña FJ, Alegre B, Gonzalez R, Castro MJ, Habing GG, Kirkwood RN. Thawing boar semen in the presence of seminal plasma: effects on sperm quality and fertility. Anim Reprod Sci 2010;119:160–5).
PB Elsevier
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10612/10668
UL http://hdl.handle.net/10612/10668
NO Theriogenology, 2013, vol. 80, n. 4
NO P. 400-410
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-abr-2024