RT info:eu-repo/semantics/article
T1 Sperm concentration at freezing affects post-thaw quality and fertility of ram semen
A1 Álvarez García, Mercedes
A1 Tamayo Canul, Julio Renan
A1 Anel Rodríguez, Enrique
A1 Boixo, Juan C.
A1 Mata Campuzano, María
A1 Martínez Pastor, Felipe
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Ram
K1 Sperm cryopreservation
K1 Sperm concentration
K1 Sperm quality
K1 Fertility
AB We have investigated the effect of sperm concentration in the freezing doses 200, 400, 800, and 1600 × 106 mL−1 on the post-thaw quality and fertility of ram semen. Semen was collected from seven adult Churra rams by artificial vagina during the breeding season. The semen was diluted in an extender (TES-Tris-fructose, 20% egg yolk, and 4% glycerol), to a final concentration of 200, 400, 800, or 1600 × 106 mL−1 and frozen. Doses were analyzed post-thawing for motility (computer-assisted sperm analysis system [CASA]), viability, and acrosomal status (fluorescence probes propidium iodide [PI]/peanut agglutinin conjugated with fluorescein thiocyanate (PNA-FITC), SYBR-14/PI [Invitrogen; Barcelona, Spain] and YO-PRO-1/PI [Invitrogen; Barcelona, Spain]). Total motility and velocity were lower for 1600 × 106 mL−1 doses, while progressive motility and viability were lower both for 800 and 1600 × 106 mL−1. The proportion of viable spermatozoa showing increased membrane permeability (YO-PRO-1+) rose in 800 and 1200 × 106 mL−1. Intrauterine inseminations were performed with the 200, 400, and 800 × 106 mL−1 doses at a fixed sperm number (25 × 106 per uterine horn) in synchronized ewes. Fertility (lambing rate) was similar for semen frozen at 200 (57.5%) or 400 × 106 mL−1 (54.4%), whereas it was significantly lower for 800 × 106 mL−1 (45.5%). In conclusion, increasing sperm concentration in cryopreserved semen, at least at 800 × 106 mL−1 and more, adversely affects the postthawing quality and fertility of ram semen.
PB Elsevier
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10612/10675
UL http://hdl.handle.net/10612/10675
NO Theriogenology, 2012, vol. 77, n. 6
NO P. 1111-1118
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024