RT info:eu-repo/semantics/article
T1 Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes
A1 Najafi, Abouzar
A1 Taheri, Ramezan Ali
A1 Mehdipour, Mahdieh
A1 Martínez Pastor, Felipe
A1 Rouhollahi, Abbas Abbas
A1 Nourani, Mohammad Reza
A2 Biologia Celular
K1 Veterinaria
K1 Ellagic acid
K1 Liposome
K1 Rooster
K1 Sperm quality
K1 Post-thawed
AB Liposomes could improve the deliveryof substances to sperm. This study was conducted toinvestigate the effect of the antioxidant ellagic acidand ellagic acid-loaded liposomes on post-thawed spermquality in broiler breeder roosters. Semen was dilutedin Beltsville extender containing ellagic acid or ellagicacid-loaded liposomes (ellagic acid at 0 (control),0.5, 1, and 2 mM) and cryopreserved. Sperm qualitywas evaluated post-thawing: motility characteristics(Computer-Assisted Semen Analysis), membranefunctionality (HOS test), abnormal morphology, mitochondrialactivity (Rhodamine 123), apoptotic status(Annexin V/Propidium iodide), malondialdehyde, andantioxidant activities (glutathione peroxidase (GPx),superoxide dismutase (SOD), and total antioxidantcapacity (TAC)). The results showed that 1 mM ellagicacid-loaded liposomes improved total motility, membranefunctionality, and viability comparing to 0.5 and2 mM ellagic acid, 2 mM ellagic acid-loaded liposomes,and control group. Mitochondrial activity was significantlyhigher for 1 mM ellagic acid-loaded liposomescompared to the rest of the treatments, except 1 mMellagic acid. Ellagic acid at 1 mM in both forms significantlyincreased GPx and TAC after freeze-thawing(no significant variation for SOD), and also yielded thelower proportion of apoptotic and dead cells. In conclusion,ellagic acid improved post-thawed sperm qualityin broiler breeder roosters. The use of liposomes couldfurther enhance the effects of ellagic acid.
PB Oxford University Press
YR 2019
FD 2019-05-13
LK http://hdl.handle.net/10612/10721
UL http://hdl.handle.net/10612/10721
NO Poultry Science, 2019,  vol. 98, n. 1
NO P. 440–446
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-abr-2024