RT info:eu-repo/semantics/article
T1 Preliminary assessment of VIS-NIR-SWIR spectroscopy with a portable instrument for the detection of Staphylococcus aureus biofilms on surfaces
A1 Fernández, Lucía
A1 Allende-Prieto, Cristina
A1 Peón, Juanjo
A1 Recondo, Carmen
A1 Rodríguez Gonzálvez, Pablo
A1 Gutíerrez, Diana
A1 Martínez, Beatriz
A1 García, Pilar
A1 Rodríguez, Ana
A2 Ingeniería CartograficaGeodesica y Fotogrametria
K1 Ingenierías
K1 Biofilms
K1 Spectroscopy
K1 Detection
K1 Food industry
AB Bacterial biofilms constitute a major source of sanitary problems and economic losses in the food industry. Indeed, biofilm removal may require intense mechanical cleaning procedures or very high concentrations of disinfectants or both, which can be damaging to the environment and human health. This study assessed the efficacy of a technique based on spectroscopy in the visible, near-infrared, and short-wavelength infrared range for the quick detection of biofilms formed on polystyrene by the pathogenic bacterium Staphylococcus aureus. To do that, biofilms corresponding to three S. aureus strains, which differed in biofilm-forming ability and composition of the extracellular matrix, were allowed to develop for 5 or 24 h, representing an active formation stage and mature biofilms, respectively. Spectral analysis of the samples, corresponding to three biological replicates of each condition, was then performed by using a portable device. The results of these experiments showed that partial least squares discriminant analysis of the spectral profile could discriminate between surfaces containing attached bacterial biomass and non-inoculated ones. In this model, the two first principal components accounted for 39 and 19% of the variance and the estimated error rate stabilized after four components. Cross-validation accuracy of this assessment was 100%. This work lays the foundation for subsequent development of a spectroscopy-based protocol that allows biofilm detection on food industrial surfaces
PB International Association for Food Protection
SN 0362-028X
LK http://hdl.handle.net/10612/12354
UL http://hdl.handle.net/10612/12354
NO 6 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 01-may-2024